Glycation and Advanced Glycation End-Products in Laboratory Experiments in Vivo and in Vitro
Martin Beránek,
Daniela Nováková,
Pavel Rozsíval,
Jaroslav Dršata,
Vladimír Palička
Affiliations
Martin Beránek
Charles University in Prague, Faculty of Medicine and University Hospital in Hradec Králové, Institute for Clinical Biochemistry and Diagnostics, Hradec Králové, Czech Republic
Daniela Nováková
Charles University in Prague, Faculty of Medicine and University Hospital in Hradec Králové, Department of Ophthalmology, Hradec Králové, Czech Republic
Pavel Rozsíval
Charles University in Prague, Faculty of Medicine and University Hospital in Hradec Králové, Department of Ophthalmology, Hradec Králové, Czech Republic
Jaroslav Dršata
Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Department of Biochemical Sciences, Hradec Králové, Czech Republic
Vladimír Palička
Charles University in Prague, Faculty of Medicine and University Hospital in Hradec Králové, Institute for Clinical Biochemistry and Diagnostics, Hradec Králové, Czech Republic
The purpose of our study was to determine the amount of glycated proteins and advanced glycation end products (AGE) in cataractous lens homogenates of patients who underwent phacoemulsification, and to define a simple in vitro protein model of glycoxidation. Analysis of 30 cataractous lenses (15 diabetic and 15 non-diabetic) revealed a significant increase in both glycated lens proteins of diabetics compared with the controls (0.15 vs 0.08 nmol/mg protein, P 25 °C > 4 °C. ALT and AST incubated in a medium containing D-fructose are subject to nonenzymatic glycation followed by a consequent formation of AGE products. Our data: i) support the concept of glycation-glycoxidation pathway appearing in diabetic patients; ii) form a base for determination of the efficiency of various antioxidative compounds in vitro.
