Rapid identification of main antibacterial components from New Zealand ‘Hass’ avocado peel hexane extract

Abstract

‘Hass’ avocado (Persea americana Mill) is a very popular tropical fruit that contains several nutrients, including vitamins, proteins, minerals, and unsaturated fatty acids. ‘Hass’ avocado peel is one of the by-products obtained from the New Zealand (NZ) avocado processing industry. The current study focused on the antibacterial components from hexane ‘Hass’ avocado peel extract. The aim was to perform microbiological screening of NZ hexane ‘Hass’ avocado peel extract using Staphylococcus aureus. We used thin- layer chromatography (TLC) with direct microbiological detection to isolate the individual components that showed antibacterial activity. Mass spectrometry (MS) analysis characterized the isolated components from hexane peel extract. (15Z)-9,12,13-Trihydroxy-15-octadecenoic acid, (±)9-HpODE, and NP-011548 are the three fatty acids that have been tentatively identified. Present findings provide new bioactive components from avocado peel and suggest potential applications for this extract as a natural antibacterial additive in soaps, and detergents.

Keywords

• New Zealand
• ‘Hass’ avocado
• Hexane peel extract
• TLC-bioautography
• LC-MS
• Compound Discoverer 3.3