Hollow CeO2 with ROS-Scavenging Activity to Alleviate Colitis in Mice

Abstract

Jing Yang,1,* Jinzhe Zhou,2,* Yingying Zhao,1 Liangchen Zhu,2 Guanghong Luo,3 BuJun Ge2 1Department of Gastroenterology, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Jinan, People’s Republic of China; 2Department of General Surgery, Tongji Hospital, Tongji University School of Medicine, Shanghai, People’s Republic of China; 3Department of Radiation Oncology, Shenzhen People’s Hospital (The Second Clinical Medical College, Jinan University, The First Affiliated Hospital, Southern University of Science and Technology), Shenzhen, People’s Republic of China*These authors contributed equally to this workCorrespondence: Guanghong Luo; Bujun Ge Email [email protected]; [email protected]: Excessive production of reactive oxygen species (ROS) to induce high oxidative stress is one of the main causes of colitis; thus, it has been regarded as a therapeutic target for colitis treatment. And the nanomaterial-based therapeutic strategies are effective against colitis. However, the previous elaborately designed materials exhibit limited application due to the uncertain biocompatibility and complicated manufacturing processes.Methods: In this study, the highly monodisperse hollow CeO2 nanoparticles (H-CeO2) with uniform morphology were obtained by in situ growing CeO2 on solid silica nanoparticles and subsequently removing the silica core. The H-CeO2 was further modified with PEG, which owned excellent biological stability and biocompatibility. The experimental model of colitis induced by dextran sulfate sodium (DSS) was used to investigate the anti-inflammatory effect of H-CeO2-PEG.Results: The H-CeO2-PEG showed good ROS scavenging efficacy and decreased the levels of proinflammatory cytokines (IL-6, IL-1β, IL-18, and TNF-α) in DSS-induced colitis mice. Furthermore, H-CeO2-PEG inhibited the activation of the MAPK signalling pathway to alleviate colitis.Conclusion: This study reveals the therapeutic effects of CeO2-based nanomedicine toward colitis and elucidates the specific signalling pathway involved, which provides potential alternative therapeutic options for patients with inflammation tissue.Keywords: hollow CeO2, ROS, inflammation, colitis, MAPK signalling pathway

Keywords

• hollow ceo2
• ros
• inflammation
• colitis
• mapk signalling pathway