Impact of Plasmonic Nanoparticles on Poikilocytosis and Microrheological Properties of Erythrocytes
Tatiana Avsievich,
Ruixue Zhu,
Alexey P. Popov,
Alexander Yatskovskiy,
Anton A. Popov,
Gleb Tikhonowsky,
Andrei I. Pastukhov,
Sergei Klimentov,
Alexander Bykov,
Andrei Kabashin,
Igor Meglinski
Affiliations
Tatiana Avsievich
Optoelectronics and Measurement Techniques, University of Oulu, 90570 Oulu, Finland
Ruixue Zhu
Optoelectronics and Measurement Techniques, University of Oulu, 90570 Oulu, Finland
Alexey P. Popov
VTT Technical Research Centre of Finland, Kaitovayla 1, 90590 Oulu, Finland
Alexander Yatskovskiy
Department of Histology, Cytology and Embryology, Institute of Clinical Medicine N.V. Sklifosovsky, I.M. Sechenov First Moscow State Medical University, Trubetskaya Street 8, 119991 Moscow, Russia
Anton A. Popov
Institute of Engineering Physics for Biomedicine (PhysBio), National Research Nuclear University (MEPhI), Kashirskoe Shosse, 31, 115409 Moscow, Russia
Gleb Tikhonowsky
Institute of Engineering Physics for Biomedicine (PhysBio), National Research Nuclear University (MEPhI), Kashirskoe Shosse, 31, 115409 Moscow, Russia
Andrei I. Pastukhov
CNRS, LP3, Aix-Marseille University, 163 Av. de Luminy, 13009 Marseille, France
Sergei Klimentov
Institute of Engineering Physics for Biomedicine (PhysBio), National Research Nuclear University (MEPhI), Kashirskoe Shosse, 31, 115409 Moscow, Russia
Alexander Bykov
Optoelectronics and Measurement Techniques, University of Oulu, 90570 Oulu, Finland
Andrei Kabashin
Institute of Engineering Physics for Biomedicine (PhysBio), National Research Nuclear University (MEPhI), Kashirskoe Shosse, 31, 115409 Moscow, Russia
Igor Meglinski
Optoelectronics and Measurement Techniques, University of Oulu, 90570 Oulu, Finland
Plasmonic nanoparticles (NP) possess great potential in photothermal therapy and diagnostics. However, novel NP require a detailed examination for potential toxicity and peculiarities of interaction with cells. Red blood cells (RBC) are important for NP distribution and the development of hybrid RBC-NP delivery systems. This research explored RBC alterations induced by noble (Au and Ag) and nitride-based (TiN and ZrN) laser-synthesized plasmonic NP. Optical tweezers and conventional microscopy modalities indicated the effects arising at non-hemolytic levels, such as RBC poikilocytosis, and alterations in RBC microrheological parameters, elasticity and intercellular interactions. Aggregation and deformability significantly decreased for echinocytes independently of NP type, while for intact RBC, all NP except Ag NP increased the interaction forces but had no effect on RBC deformability. RBC poikilocytosis promoted by NP at concentration 50 μg mL−1 was more pronounced for Au and Ag NP, compared to TiN and ZrN NP. Nitride-based NP demonstrated better biocompatibility towards RBC and higher photothermal efficiency than their noble metal counterparts.
