PLoS ONE (Jan 2020)

In vitro hemo- and cytocompatibility of bacterial nanocelluose small diameter vascular grafts: Impact of fabrication and surface characteristics.

  • Max Wacker,
  • Viktoria Kießwetter,
  • Ingo Slottosch,
  • George Awad,
  • Adnana Paunel-Görgülü,
  • Sam Varghese,
  • Maurice Klopfleisch,
  • Dennis Kupitz,
  • Dieter Klemm,
  • Sandor Nietzsche,
  • Katrin Petzold-Welcke,
  • Friederike Kramer,
  • Jens Wippermann,
  • Priya Veluswamy,
  • Maximilian Scherner

DOI
https://doi.org/10.1371/journal.pone.0235168
Journal volume & issue
Vol. 15, no. 6
p. e0235168

Abstract

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ObjectiveThere is an increasing need for small diameter vascular grafts with superior host hemo- and cytocompatibilities, such as low activation of platelets and leukocytes. Therefore, we aimed to investigate whether the preparation of bacterial nanocellulose grafts with different inner surfaces has an impact on in vitro host cytocompatibility.MethodsWe have synthesized five different grafts in a bioreactor, namely open interface surface (OIS), inverted (INV), partially air dried (PAD), surface formed in air contact (SAC) and standard (STD) that were characterized by a different surface roughness. The grafts (length 55 mm, inner diameter 5 mm) were attached to heparinized polyvinyl chloride tubes, loaded with human blood and rotated at 37°C for 4 hours. Then, blood was analyzed for frequencies of cellular fractions, oxidative products, soluble complement and thrombin factors. The results were compared to clinically approved grafts made of polyethylene terephthalate and expanded polytetrafluoroethylene. Additionally, blood platelets were labelled with 111Indium-oxine to visualize the distribution of adherent platelets in the loop by scintigraphy.ResultsSAC nanocellulose grafts with the lowest surface roughness exhibited superior performance with 65% leukocyte and >90% thrombocyte loss. Of note, SAC nanocellulose grafts showed lowest radioactivity with scintigraphy analyses, indicating reduced platelet adhesion. Although the levels of reactive oxygen species and cell free DNA did not differ significantly, the levels of thrombin-antithrombin complexes were lowest in SAC grafts. However, all nanocellulose grafts exhibited enhanced complement activation.ConclusionThe systematic variation of the inner surfaces of BNC vascular grafts significantly improves biocompatibility. Especially, SAC grafts exhibited the lowest loss of platelets as well as leukocytes and additionally significantly diminished activation of the coagulation system. Further animal studies are needed to study in vivo biocompatibilities.