Nature Communications (Sep 2023)
Single-cell transcriptome analysis indicates fatty acid metabolism-mediated metastasis and immunosuppression in male breast cancer
- Handong Sun,
- Lishen Zhang,
- Zhonglin Wang,
- Danling Gu,
- Mengyan Zhu,
- Yun Cai,
- Lu Li,
- Jiaqi Tang,
- Bin Huang,
- Bakwatanisa Bosco,
- Ning Li,
- Lingxiang Wu,
- Wei Wu,
- Liangyu Li,
- Yuan Liang,
- Lin Luo,
- Quanzhong Liu,
- Yanhui Zhu,
- Jie Sun,
- Liang Shi,
- Tiansong Xia,
- Chuang Yang,
- Qitong Xu,
- Xue Han,
- Weiming Zhang,
- Jianxia Liu,
- Dong Meng,
- Hua Shao,
- Xiangxin Zheng,
- Shuqin Li,
- Hua Pan,
- Jing Ke,
- Wenying Jiang,
- Xiaolan Zhang,
- Xuedong Han,
- Jian Chu,
- Hongyin An,
- Juyan Ge,
- Chi Pan,
- Xiuxing Wang,
- Kening Li,
- Qianghu Wang,
- Qiang Ding
Affiliations
- Handong Sun
- Jiangsu Breast Disease Center, The First Affiliated Hospital with Nanjing Medical University
- Lishen Zhang
- Department of Bioinformatics, Nanjing Medical University
- Zhonglin Wang
- Department of Breast Surgery, The Second People’s Hospital of Lianyungang
- Danling Gu
- National Health Commission Key Laboratory of Antibody Techniques, Department of Cell Biology, Jiangsu Provincial Key Laboratory of Human Functional Genomics, School of Basic Medical Sciences, Nanjing Medical University
- Mengyan Zhu
- Department of Bioinformatics, Nanjing Medical University
- Yun Cai
- Department of Bioinformatics, Nanjing Medical University
- Lu Li
- Department of Bioinformatics, Nanjing Medical University
- Jiaqi Tang
- Department of Bioinformatics, Nanjing Medical University
- Bin Huang
- Department of Bioinformatics, Nanjing Medical University
- Bakwatanisa Bosco
- Department of Bioinformatics, Nanjing Medical University
- Ning Li
- Department of Bioinformatics, Nanjing Medical University
- Lingxiang Wu
- Department of Bioinformatics, Nanjing Medical University
- Wei Wu
- Department of Bioinformatics, Nanjing Medical University
- Liangyu Li
- Department of Bioinformatics, Nanjing Medical University
- Yuan Liang
- Department of Bioinformatics, Nanjing Medical University
- Lin Luo
- Department of Bioinformatics, Nanjing Medical University
- Quanzhong Liu
- Department of Bioinformatics, Nanjing Medical University
- Yanhui Zhu
- Jiangsu Breast Disease Center, The First Affiliated Hospital with Nanjing Medical University
- Jie Sun
- Department of Breast Surgery, The First Affiliated Hospital of Soochow University
- Liang Shi
- Jiangsu Breast Disease Center, The First Affiliated Hospital with Nanjing Medical University
- Tiansong Xia
- Jiangsu Breast Disease Center, The First Affiliated Hospital with Nanjing Medical University
- Chuang Yang
- Jiangsu Breast Disease Center, The First Affiliated Hospital with Nanjing Medical University
- Qitong Xu
- Jiangsu Breast Disease Center, The First Affiliated Hospital with Nanjing Medical University
- Xue Han
- Department of Pathology, The First Affiliated Hospital with Nanjing Medical University
- Weiming Zhang
- Department of Pathology, The First Affiliated Hospital with Nanjing Medical University
- Jianxia Liu
- Department of Breast Surgery, The First Affiliated Hospital of Soochow University
- Dong Meng
- Department of Breast Surgery, Affiliated Hospital of Jiangnan University
- Hua Shao
- Department of Breast Surgery, The Second People’s Hospital of Lianyungang
- Xiangxin Zheng
- Department of Breast Surgery, Affiliated Suqian Hospital of Xuzhou Medical University
- Shuqin Li
- The Affiliated Lianyungang Hospital of Xuzhou Medical University
- Hua Pan
- Liyang People’s Hospital
- Jing Ke
- The Affiliated Hospital of Nantong University
- Wenying Jiang
- Department of Breast Surgery, The Third Affiliated Hospital of Soochow University
- Xiaolan Zhang
- Department of Breast Surgery, The Affiliated Changzhou Second People’s Hospital of Nanjing Medical University
- Xuedong Han
- Department of Breast and Thyroid Surgery, Huai’an First People’s Hospital, Nanjing Medical University
- Jian Chu
- Department of General Surgery, the First People’s Hospital of Yancheng
- Hongyin An
- Department of General Surgery, the First People’s Hospital of Yancheng
- Juyan Ge
- Department of Pathology, The Second People’s Hospital of Lianyungang
- Chi Pan
- Department of Breast Surgery, the Second Affiliated Hospital, Zhejiang University, College of Medicine
- Xiuxing Wang
- National Health Commission Key Laboratory of Antibody Techniques, Department of Cell Biology, Jiangsu Provincial Key Laboratory of Human Functional Genomics, School of Basic Medical Sciences, Nanjing Medical University
- Kening Li
- Department of Bioinformatics, Nanjing Medical University
- Qianghu Wang
- Department of Bioinformatics, Nanjing Medical University
- Qiang Ding
- Jiangsu Breast Disease Center, The First Affiliated Hospital with Nanjing Medical University
- DOI
- https://doi.org/10.1038/s41467-023-41318-2
- Journal volume & issue
-
Vol. 14,
no. 1
pp. 1 – 18
Abstract
Abstract Male breast cancer (MBC) is a rare but aggressive malignancy with cellular and immunological characteristics that remain unclear. Here, we perform transcriptomic analysis for 111,038 single cells from tumor tissues of six MBC and thirteen female breast cancer (FBC) patients. We find that that MBC has significantly lower infiltration of T cells relative to FBC. Metastasis-related programs are more active in cancer cells from MBC. The activated fatty acid metabolism involved with FASN is related to cancer cell metastasis and low immune infiltration of MBC. T cells in MBC show activation of p38 MAPK and lipid oxidation pathways, indicating a dysfunctional state. In contrast, T cells in FBC exhibit higher expression of cytotoxic markers and immune activation pathways mediated by immune-modulatory cytokines. Moreover, we identify the inhibitory interactions between cancer cells and T cells in MBC. Our study provides important information for understanding the tumor immunology and metabolism of MBC.
