Current challenges and solutions of super-resolution structured illumination
microscopy
Xiaomin Zheng,
Jie Zhou,
Lei Wang,
Meiting Wang,
Wenshuai Wu,
Jiajie Chen,
Junle Qu,
Bruce Zhi Gao,
Yonghong Shao
Affiliations
Xiaomin Zheng
College of Physics and Optoelectronic Engineering,
Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and
Guangdong Province, Shenzhen University, Shenzhen 518060,
China
Jie Zhou
College of Physics and Optoelectronic Engineering,
Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and
Guangdong Province, Shenzhen University, Shenzhen 518060,
China
Lei Wang
College of Physics and Optoelectronic Engineering,
Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and
Guangdong Province, Shenzhen University, Shenzhen 518060,
China
Meiting Wang
College of Physics and Optoelectronic Engineering,
Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and
Guangdong Province, Shenzhen University, Shenzhen 518060,
China
Wenshuai Wu
College of Physics and Optoelectronic Engineering,
Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and
Guangdong Province, Shenzhen University, Shenzhen 518060,
China
Jiajie Chen
College of Physics and Optoelectronic Engineering,
Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and
Guangdong Province, Shenzhen University, Shenzhen 518060,
China
Junle Qu
College of Physics and Optoelectronic Engineering,
Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and
Guangdong Province, Shenzhen University, Shenzhen 518060,
China
Bruce Zhi Gao
Department of Bioengineering, Clemson
University, Clemson, South Carolina 29634, USA
Yonghong Shao
College of Physics and Optoelectronic Engineering,
Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and
Guangdong Province, Shenzhen University, Shenzhen 518060,
China
The resolution of fluorescence microscopy is limited by the diffraction imaging system, and many methods have been proposed to overcome the optical diffraction limit for achieving super-resolution imaging. Structured illumination microscopy (SIM) is one of the most competitive approaches and has demonstrated remarkable achievements. In the last two decades, SIM has been improved in many aspects, such as the enhancement of resolution and imaging depth and virtual modulation-based SIM. In this Perspective, we present an overview of the development of SIM, including the basic theory, application to biomedical studies, and the remarkable progress of SIM. Owing to its flexibility with respect to combination with other methods, SIM can be considered a powerful tool for biomedical study, offering augmented imaging capabilities by exploiting complementary advantages.
