A prognostic signature based on snoRNA predicts the overall survival of lower-grade glioma patients

Yi Zhou; Yi Zhou; Wen Yin; Wen Yin; Yirui Kuang; Yirui Kuang; Zhaoping Wu; Zhaoping Wu; Haoxuan Huang; Haoxuan Huang; Weidong Liu; Weidong Liu; Weidong Liu; Weidong Liu; Xingjun Jiang; Xingjun Jiang; Caiping Ren; Caiping Ren; Caiping Ren; Caiping Ren

doi:10.3389/fimmu.2023.1138363

Frontiers in Immunology (Nov 2023)

A prognostic signature based on snoRNA predicts the overall survival of lower-grade glioma patients

Yi Zhou,
Yi Zhou,
Wen Yin,
Wen Yin,
Yirui Kuang,
Yirui Kuang,
Zhaoping Wu,
Zhaoping Wu,
Haoxuan Huang,
Haoxuan Huang,
Weidong Liu,
Weidong Liu,
Weidong Liu,
Weidong Liu,
Xingjun Jiang,
Xingjun Jiang,
Caiping Ren,
Caiping Ren,
Caiping Ren,
Caiping Ren

Affiliations

Yi Zhou: Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha, Hunan, China
Yi Zhou: National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, Hunan, China
Wen Yin: Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha, Hunan, China
Wen Yin: National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, Hunan, China
Yirui Kuang: Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha, Hunan, China
Yirui Kuang: National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, Hunan, China
Zhaoping Wu: Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha, Hunan, China
Zhaoping Wu: National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, Hunan, China
Haoxuan Huang: Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha, Hunan, China
Haoxuan Huang: National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, Hunan, China
Weidong Liu: Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha, Hunan, China
Weidong Liu: National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, Hunan, China
Weidong Liu: Cancer Research Institute, School of Basic Medical Science, Central South University, Changsha, China
Weidong Liu: The NHC Key Laboratory of Carcinogenesis and The Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Central South University, Changsha, Hunan, China
Xingjun Jiang: Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha, Hunan, China
Xingjun Jiang: National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, Hunan, China
Caiping Ren: Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha, Hunan, China
Caiping Ren: National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, Hunan, China
Caiping Ren: Cancer Research Institute, School of Basic Medical Science, Central South University, Changsha, China
Caiping Ren: The NHC Key Laboratory of Carcinogenesis and The Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Central South University, Changsha, Hunan, China

DOI: https://doi.org/10.3389/fimmu.2023.1138363
Journal volume & issue: Vol. 14

Abstract

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IntroductionSmall nucleolar RNAs (snoRNAs) are a group of non-coding RNAs enriched in the nucleus which direct post-transcriptional modifications of rRNAs, snRNAs and other molecules. Recent studies have suggested that snoRNAs have a significant role in tumor oncogenesis and can be served as prognostic markers for predicting the overall survival of tumor patients. MethodsWe screened 122 survival-related snoRNAs from public databases and eventually selected 7 snoRNAs that were most relevant to the prognosis of lower-grade glioma (LGG) patients for the establishment of the 7-snoRNA prognostic signature. Further, we combined clinical characteristics related to the prognosis of glioma patients and the 7-snoRNA prognostic signature to construct a nomogram.ResultsThe prognostic model displayed greater predictive power in both validation set and stratification analysis. Results of enrichment analysis revealed that these snoRNAs mainly participated in the post-transcriptional process such as RNA splicing, metabolism and modifications. In addition, 7-snoRNA prognostic signature were positively correlated with immune scores and expression levels of multiple immune checkpoint molecules, which can be used as potential biomarkers for immunotherapy prediction. From the results of bioinformatics analysis, we inferred that SNORD88C has a major role in the development of glioma, and then performed in vitro experiments to validate it. The results revealed that SNORD88C could promote the proliferation, invasion and migration of glioma cells. DiscussionWe established a 7-snoRNA prognostic signature and nomogram that can be applied to evaluate the survival of LGG patients with good sensitivity and specificity. In addition, SNORD88C could promote the proliferation, migration and invasion of glioma cells and is involved in a variety of biological processes related to DNA and RNA.

Published in Frontiers in Immunology

ISSN: 1664-3224 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Medicine: Internal medicine: Specialties of internal medicine: Immunologic diseases. Allergy
Website: http://journal.frontiersin.org/journal/immunology

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