BMC Veterinary Research (Apr 2025)

Development of an in-house indirect ELISA kit for the serological detection of antibodies against infectious bursal disease in chickens

  • Takele Tesgera Hurisa,
  • Takele Abayneh,
  • Teshale Sori,
  • Guohua Chengh,
  • Yongxiang Fang,
  • Berecha Bayisa,
  • Mirtneh Akalu,
  • Kedir Sherefa,
  • Getu Ayele,
  • Adugna Geresu,
  • Hunduma Dinka,
  • Mulatu Mokonnin,
  • Warkisa Chala Bulto,
  • Teferi Degefa Negi,
  • Berhan Demeke,
  • Megersa Mindaye Yami,
  • Mohammed Jemal,
  • Abinet Legesse,
  • Yeneneh Tesfaye,
  • Amde Zeleke

DOI
https://doi.org/10.1186/s12917-025-04756-2
Journal volume & issue
Vol. 21, no. 1
pp. 1 – 8

Abstract

Read online

Abstract Infectious bursal disease is a highly contagious disease of young chickens caused by the infectious bursal disease virus. This disease poses an important threat to the commercial poultry industry globally. This study was designed to develop an In-House Indirect Enzyme-Linked Immune Sorbent Assay Kit for the serological detection of antibodies against infectious bursal disease viruses. An infectious bursal disease virus antigen dilution (1:2), sample serum (1:500), and mouse anti-chicken immunoglobulin G (IgG) labeled with horseradish peroxidase (HRP) (1:2,000) were used in this assay. The calculated cutoff value was 0.24. This in-house indirect ELISA method was compared with a commercial ELISA kit for the detection of antibodies against infectious bursal disease virus in chickens. The performance of the newly developed and commercial ELISA kit was evaluated as described by Samad et al. (1994). The sensitivity and specificity of the current ELISA method were 98% (95% CI: 92.96–99.76) and 97% (95% CI: 91.48–99.38), respectively. The average intra-assay % CV of the triplet of 2 samples was 7.6, and interassay comparisons indicated a CV of 5.45%. As indicated by the results, we described a valuable and cost-effective, sensitive and specific in-house indirect ELISA kit for the serological diagnosis of infectious bursal disease in Ethiopia.

Keywords