Efficient population modification gene-drive rescue system in the malaria mosquito Anopheles stephensi

Adriana Adolfi; Valentino M. Gantz; Nijole Jasinskiene; Hsu-Feng Lee; Kristy Hwang; Gerard Terradas; Emily A. Bulger; Arunachalam Ramaiah; Jared B. Bennett; J. J. Emerson; John M. Marshall; Ethan Bier; Anthony A. James

doi:10.1038/s41467-020-19426-0

Nature Communications (Nov 2020)

Efficient population modification gene-drive rescue system in the malaria mosquito Anopheles stephensi

Adriana Adolfi,
Valentino M. Gantz,
Nijole Jasinskiene,
Hsu-Feng Lee,
Kristy Hwang,
Gerard Terradas,
Emily A. Bulger,
Arunachalam Ramaiah,
Jared B. Bennett,
J. J. Emerson,
John M. Marshall,
Ethan Bier,
Anthony A. James

Affiliations

Adriana Adolfi: Department of Microbiology & Molecular Genetics, University of California
Valentino M. Gantz: Section of Cell and Developmental Biology, University of California, San Diego
Nijole Jasinskiene: Department of Microbiology & Molecular Genetics, University of California
Hsu-Feng Lee: Department of Microbiology & Molecular Genetics, University of California
Kristy Hwang: Department of Microbiology & Molecular Genetics, University of California
Gerard Terradas: Section of Cell and Developmental Biology, University of California, San Diego
Emily A. Bulger: Section of Cell and Developmental Biology, University of California, San Diego
Arunachalam Ramaiah: Department of Ecology and Evolutionary Biology, University of California
Jared B. Bennett: Biophysics Graduate Group, Division of Biological Sciences, College of Letters and Science, University of California
J. J. Emerson: Department of Ecology and Evolutionary Biology, University of California
John M. Marshall: Division of Epidemiology & Biostatistics, School of Public Health, University of California
Ethan Bier: Section of Cell and Developmental Biology, University of California, San Diego
Anthony A. James: Department of Microbiology & Molecular Genetics, University of California

DOI: https://doi.org/10.1038/s41467-020-19426-0
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 13

Abstract

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Gene drives may be impeded by the generation of resistant alleles following NHEJ. Here the authors develop a recoded gene-drive rescue system for the malaria mosquito, Anopheles stephensi, that targets the drive to the kynurenine hydroxylase gene for negative selection against mutated alleles.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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