Effect of Egg White Combined with Chalcanthite on Lipopolysaccharide induced Inflammatory Cytokine Expression in RAW 264.7 cells

Abstract

Historically, mineral compound herbal medicines have long been used in treatments of immune-related diseases in Korea, China and other Asian countries. In this study, we inv-estigated the anti-inflammatory effect of egg white combined with chalcanthite (IS4) on lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. RAW 264.7 cells cultured with LPS and various con-centrations of IS4 were analyzed to determine the production of pro-inflammatory cytokines and mediators by using enzyme-linked immune sorbent assays (ELISAs). IS4 concentration inhibited the production of interleukin-1beta (IL-1β), interleukin-6 (IL-6), and granulocyte-macrophage colony-stimulating factor (GM-CSF) induced by LPS. IS4 at high concentrations (25 and 50`㎍/ml) inhibited, in concentration-dependent manner, the expression of tumor necrosis factor-alpha (TNF–α) stimulated by LPS. IS4 has shown an anti-inflammatory effect in RAW 264.7 cells.

Keywords

• cytokine
• GM-CSF
• IL-1β
• IL-6
• lipopolysaccharide
• TNF–α