EFFECT OF ANTIFUNGAL TREFLAN AND LENTIL ESSENTIAL OIL DISC INHIBITION METHODS ON LAGENIDIUM CALLINECTES

Abstract

Lagenidium callinectes is marine phycomycetous parasitic fungus capable to spread disease in marine Crustacea eggs and larvae. Treflan (trifluralin, Elanco) is used in penaeid shrimp larval mycosis treatment. The disease was caused by the phycomycetous fungi Lagenidium sp. and Sirolpidium sp. The study aimed to determine the efficacy of Treflan and Lentil Essential Oil to Lagenidium callinectes growth inhibition in Potatoes Dextrose Agar (PDA) + 2% NaCl. This study was observational analytical research utilizing experimental design. The data were obtained from two treatment group. Treatment Group 1 used Lentil Essential Oil and Treatment Group 2 used Treflan product. Each treatment dilution 10-1, 10-2, 10-3, 10-4, 10-5, 10-6 were dripped onto disc. The control group was immersed in sterile seawater. The inhibition zone diameters from each plate were measured. The treflan treatment group exhibited Lagenidium sp. 11.08 cm growth inhibition zone in the second day of incubation and 8.27 cm growth inhibition zone in the third day of incubation on dilution 10-1 (100.000 ppm). Lentil Essential Oil treatment group exhibited Lagenidium sp 8.38 cm growth inhibition zone on the second day of incubation on dilution 10-1 (100000 ppm). The study indicated that Treflan and Lentil Essential Oils are capable to inhibit Lagenidium sp. growth as these contain antifungal compounds.

Keywords

• Lagenidium sp.
• Treflan product
• lentil essential oil
• growth inhibition