Exploring Reactive Oxygen Species Accumulation in <i>Allium fistulosum</i> L. Seeds Exposed to Different Storage Conditions
Gregorio Padula,
Anca Macovei,
Adriano Ravasio,
Andrea Pagano,
Conrado Jr Dueñas,
Xianzong Xia,
Roman Hołubowicz,
Alma Balestrazzi
Affiliations
Gregorio Padula
Department of Plant Pathology, Seed Science and Technology, Faculty of Agriculture, Horticulture and Bioengineering, Poznan University of Life Sciences, 60-637 Poznan, Poland
Anca Macovei
Department of Biology and Biotechnology ‘L. Spallanzani’, University of Pavia, Via Ferrata 1, 27100 Pavia, Italy
Adriano Ravasio
NeoruraleHub, Innovation Center Giulio Natta, Località Cascina Darsena, Giussago, 27010 Pavia, Italy
Andrea Pagano
Department of Biology and Biotechnology ‘L. Spallanzani’, University of Pavia, Via Ferrata 1, 27100 Pavia, Italy
Conrado Jr Dueñas
Department of Biology and Biotechnology ‘L. Spallanzani’, University of Pavia, Via Ferrata 1, 27100 Pavia, Italy
Xianzong Xia
Department of Plant Pathology, Seed Science and Technology, Faculty of Agriculture, Horticulture and Bioengineering, Poznan University of Life Sciences, 60-637 Poznan, Poland
Roman Hołubowicz
Department of Plant Pathology, Seed Science and Technology, Faculty of Agriculture, Horticulture and Bioengineering, Poznan University of Life Sciences, 60-637 Poznan, Poland
Alma Balestrazzi
Department of Biology and Biotechnology ‘L. Spallanzani’, University of Pavia, Via Ferrata 1, 27100 Pavia, Italy
The purpose of this work was to investigate the production of reactive oxygen species (ROS) in Allium fistulosum seeds stored under different conditions. Optimized seed storage conditions are essential to maintain seed viability, otherwise accumulation of ROS-induced oxidative damage can lead to seed aging. The A. fistulosum seed lots used in this study have been selected based on their breeding background and reproduction site. Seed samples were stored up to 22 months under six different conditions of temperature (25, 10, and 7.5 °C) and relative humidity (RH) (25% and 45% RH). A germination test and ROS quantification assay were performed on the samples collected after 12 and 22 months of storage, respectively. Within a time-window of 10 months, the tested seed lots evidenced a decrease in the germination rate associated with increased ROS levels. Correlation analysis also showed that ROS production was influenced by genotype. The reported data showed that ROS accumulation was dependent on the storage condition and genotype. Some of the tested seed lots appeared to be prone to ROS accumulation, independent of storage conditions. On the other hand, specific condition storages (25 °C, 25% RH; 25 °C, 45% RH; 10 °C, 25% RH; 7.5 °C, 25% RH) resulted in a lower impact on seed aging.
