Application of nuclear technology in maternal inheritance of ship sturgeon (Acipenser nudiventris)

Abstract

Induction of Gynogenesis in sturgeon is important, therefore, the aim of this study was gynogenesis inducing in Ship sturgeon Acipenser nudiventris by gamma radiation to the heterologous sperm of Persian sturgeon Acipenser persicus. At first, sperm was extracted, and in the next stage, irradiation was performed with doses of 0.45, 0.6, 0.75, 0.9 and 1.05 kGy. Then oocytes were obtained from fish and fertilized by different dosages of irradiated sperms and cold shock was used to ploidy inducing. Control group (fertilization of normal sperm and oocytes), hybrid (fertilization of normal Persian sturgeon sperm and ship sturgeon oocytes), haploid (fertilization of irradiated sperm and normal ship oocytes), and triploid (fertilization of ship sperm and oocytes with cold temperature shock) were considered. The fertilized eggs were transferred to the incubator until hatching and the percentage of fertilization and hatching was calculated after the evolutionary period in the incubation. DNA was extracted from different group’s larvae and the success rate of inoculation was determined using Afu9 and Afu68 microsatellite markers. The results showed that the 0.9 kGy group had a higher rate of fertilization and hatching (P <0.05). Inheritance assessment showed that gynogenesis was performed successfully in different groups. It can be concluded that gynogenesis in this fish has been done successfully and due to the higher efficiency, 0.9 kGy dose, it was recommended for this species gynogenesis.

Keywords

• ship sturgeon
• gynogenesis
• gamma ray
• afu9 and afu68 sequences