Infection and Drug Resistance (Nov 2022)

NDM-1 and OXA-48-Like Carbapenemases (OXA-48, OXA-181 and OXA-252) Co-Producing Shewanella xiamenensis from Hospital Wastewater, China

  • Wen Y,
  • Xie X,
  • Xu P,
  • Yang C,
  • Zhu Z,
  • Zhu J,
  • Lv J,
  • Zhang H,
  • Chen L,
  • Du H

Journal volume & issue
Vol. Volume 15
pp. 6927 – 6938

Abstract

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Yicheng Wen,1,* Xiaofang Xie,1,* Ping Xu,2,* Chengcheng Yang,1 Zhichen Zhu,1 Jie Zhu,1 Jingnan Lv,1 Haifang Zhang,1 Liang Chen,3,4 Hong Du1 1Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, People’s Republic of China; 2Department of Clinical Laboratory, The Fifth People’s Hospital of Suzhou, Suzhou, Jiangsu, People’s Republic of China; 3Hackensack Meridian Health Center for Discovery and Innovation, Nutley, NJ, USA; 4Department of Medical Sciences, Hackensack Meridian School of Medicine, Nutley, NJ, USA*These authors contributed equally to this workCorrespondence: Hong Du, Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, 1055 Sanxiang Road, Suzhou, Jiangsu, People’s Republic of China, Email [email protected]: Shewanella genus, as an important carrier of resistance genes, has the potential to transmit resistance to many antimicrobials in many circumstances, especially in aquatic environment. The aim of the study was to describe the risk of Shewanella xiamenensis in hospital environment through analysis of genomic comparison and resistance status.Methods: Seven S. xiamenensis strains were isolated from hospital wastewater. PCR and Sanger sequencing were carried out for detection of common carbapenemase genes. Antimicrobial susceptibility testing was performed to determine the antimicrobial profile. Whole genome sequencing was applied, and sequences were further used for genomic analysis.Results: Seven Shewanella xiamenensis were all positive for blaNDM and blaOXA-48. Antimicrobial susceptibility testing showed all Shewanella xiamenensis were resistant to cefotaxime, ceftazidime, imipenem, meropenem, gentamycin and trimethoprim-sulfamethoxazole. Whole genome sequencing and phylogenetic analysis demonstrated the diversity of Shewanella xiamenensis despite isolating from one wastewater pool.Conclusion: To the best of our knowledge, this is the first report of detection of three types blaOXA-48-like genes in one hospital in China. And we have detected multi-drug resistant S. xiamenensis from hospital wastewater. This emphasizes that the presence of naturally existing carbapenemases in the environment may be significantly overlooked and that the blaOXA-48-like genes in China may originate through the horizontal gene transfer from S. xiamenensis to Enterobacterales rather than import from other countries.Keywords: OXA-48, NDM, Shewanella xiamenensis, hospital wastewater, reservoir, horizontal transmission, surveillance, New Delhi metallo-β-Lactamase

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