Acta Cirúrgica Brasileira (Mar 2017)

Influence of remote ischemic conditioning and tramadol hydrochloride on oxidative stress in kidney ischemia/reperfusion injury in rats

  • Rita de Cássia Silva de Oliveira,
  • Marcus Vinicius Henriques Brito,
  • Rubens Fernando Gonçalves Ribeiro Júnior,
  • Leonam Oliver Durval Oliveira,
  • Andrew Moraes Monteiro,
  • Fernando Mateus Viegas Brandão,
  • Lainy Carollyne da Costa Cavalcante,
  • Eduardo Henrique Herbster Gouveia,
  • Higor Yuri Bezerra Henriques

DOI
https://doi.org/10.1590/s0102-865020170030000007
Journal volume & issue
Vol. 32, no. 3
pp. 229 – 235

Abstract

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Abstract Purpose: To evaluate the effects of tramadol hydrochloride associated to remote ischemic perconditioning on oxidative stress. Methods: Twenty five male rats (Wistar) underwent right nephrectomy and were distributed into five groups: Sham group (S); Ischemia/Reperfusion group (I/R) with 30 minutes of renal ischemia; Remote ischemic perconditioning group (Per) with three cycles of 10 minutes of I/R performed during kidney ischemia; Tramadol group (T) treated with tramadol hydrochloride (40mg/kg); remote ischemic perconditioning + Tramadol group (Per+T) with both treatments. Oxidative stress was assessed after 24 hours of reperfusion. Results: Statistical differences were observed in MDA levels between I/R group with all groups (p<0.01), in addition there was difference between Tramadol with Sham, Per and Per+T groups (p<0.05), both in plasma and renal tissue. Conclusion: Remote ischemic perconditioning was more effective reducing renal ischemia-reperfusion injury than administration of tramadol or association of both treatments.

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