Marine Fungal Cerebroside Flavuside B Protects HaCaT Keratinocytes against <i>Staphylococcus aureus</i> Induced Damage
Ekaterina A. Chingizova,
Ekaterina S. Menchinskaya,
Artur R. Chingizov,
Evgeny A. Pislyagin,
Elena V. Girich,
Anton N. Yurchenko,
Irina V. Guzhova,
Valery V. Mikhailov,
Dmitry L. Aminin,
Ekaterina A. Yurchenko
Affiliations
Ekaterina A. Chingizova
Laboratory of Bioassays and Mechanism of Action of Biologically Active Substances, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Russian Academy of Sciences, Prosp. 100 Let Vladivostoku 159, Vladivostok 690022, Russia
Ekaterina S. Menchinskaya
Laboratory of Bioassays and Mechanism of Action of Biologically Active Substances, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Russian Academy of Sciences, Prosp. 100 Let Vladivostoku 159, Vladivostok 690022, Russia
Artur R. Chingizov
Laboratory of Microbiology, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Russian Academy of Sciences, Prosp. 100 Let Vladi-vostoku 159, Vladivostok 690022, Russia
Evgeny A. Pislyagin
Laboratory of Bioassays and Mechanism of Action of Biologically Active Substances, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Russian Academy of Sciences, Prosp. 100 Let Vladivostoku 159, Vladivostok 690022, Russia
Elena V. Girich
Laboratory of Chemistry of Microbial Metabolites, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Russian Academy of Sciences, Prosp. 100 Let Vladivostoku 159, Vladivostok 690022, Russia
Anton N. Yurchenko
Laboratory of Chemistry of Microbial Metabolites, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Russian Academy of Sciences, Prosp. 100 Let Vladivostoku 159, Vladivostok 690022, Russia
Irina V. Guzhova
Institute of Cytology, Russian Academy of Sciences, Tikhoretsky Ave., 4, St. Petersburg 194064, Russia
Valery V. Mikhailov
Laboratory of Microbiology, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Russian Academy of Sciences, Prosp. 100 Let Vladi-vostoku 159, Vladivostok 690022, Russia
Dmitry L. Aminin
Laboratory of Bioassays and Mechanism of Action of Biologically Active Substances, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Russian Academy of Sciences, Prosp. 100 Let Vladivostoku 159, Vladivostok 690022, Russia
Ekaterina A. Yurchenko
Laboratory of Bioassays and Mechanism of Action of Biologically Active Substances, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Russian Academy of Sciences, Prosp. 100 Let Vladivostoku 159, Vladivostok 690022, Russia
Cerebrosides are glycosylated sphingolipids, and in mammals they contribute to the pro-/anti-inflammatory properties and innate antimicrobial activity of the skin and mucosal surfaces. Staphylococcus aureus infection can develop, not only from minor scratches of the skin, but this pathogen can also actively promote epithelial breach. The effect of cerebroside flavuside B from marine sediment-derived fungus Penicillium islandicum (Aniva Bay, the Sea of Okhotsk) on viability, apoptosis, total caspase activity, and cell cycle in human epidermal keratinocytes HaCaT line co-cultivated with S. aureus, as well as influence of flavuside B on LPS-treated HaCaT cells were studied. Influence of flavuside B on bacterial growth and biofilm formation of S. aureus and its effect on the enzymatic activity of sortase A was also investigated. It was found S. aureus co-cultivated with keratinocytes induces caspase-depended apoptosis and cell death, arrest cell cycle in the G0/G1 phase, and increases in cellular immune inflammation. Cerebroside flavuside B has demonstrated its antimicrobial and anti-inflammatory properties, substantially eliminating all the negative consequences caused by co-cultivation of keratinocytes with S. aureus or bacterial LPS. The dual action of flavuside B may be highly effective in the treatment of bacterial skin lesions and will be studied in the future in in vivo experiments.
