Evaluation of expression of p16INK4a & Survivin in squamous neoplasm of cervix- A 3 years study

Abstract

Objective: To explore the expression of p16INK4a(p16) & survivin in cervical intraepithelial neoplasm (CIN) & cervical squamous neoplasm. Materials & Methods: A 3years study was undertaken in MGM Hospital, Warangal, all the cervical & hysterectomy specimens were studied. By considering both exclusion & inclusion criteria 46 cases were taken for p16INK4A & survivin expression & data was analysed. Results: The p16INK4A & survivin expressions showed the presence of statistical significance in cervical cancer, CIN1, CINII, CINIII and a normal cervical tissue & the comparison also revealed statistical significance among group. P16INK4A showed increase intensity with grade ( CINI = 28.57%, CINII = 44.44%, CINIII = 70%, & SCC = 90%), whereas survivin expressions were positive for both nuclear as well as cytoplasm staining( CINI = 28.57%, CINII = 22.22%,CINIII = 40%, SCC = 75% ). Moreover, p16INK4A protein was associated with CIN grade & in cervical cancer; survivin protein was also related with clinical stages, CIN grade; the p16INK4A & survivin expressions were positively correlated with cervical cancer, & associated with poor prognosis of cervical cancer. Conclusion: Briefly, p16INK4A & survivin expressions were positively correlated with the clinico-pathological & prognosis of cervical cancer.P16INK4A linearly correlates well with increasing grade of CIN & SCC, whereas as survivin does not shows linear correlation with increasing grades of CIN . But it was the useful marker for differentiating CIN & SCC, especially in diagnostically difficult cases

Keywords

• p16ink4a
• survivin
• carcinoma in-situ
• squamous cell carcinoma
• cervix