Food Materials Research (Feb 2023)
An aqueous-two phase extraction system for partitioning of anthocyanins from red cabbage
Abstract
Due to their aesthetic value and positive health effects, anthocyanins as natural colorants have become very attractive in recent years. There is a need to develop simple and effective methods for the separation and purification of these natural pigments. An aqueous two-phase extraction (ATPE) system based on ethanol/ammonium sulfate is employed for the extraction and preliminary purification of anthocyanins from red cabbage. The influence of ethanol, ammonium sulfate, red cabbage amount and pH on the extraction of anthocyanins is investigated. The optimal extraction conditions (27% (v/v) ethanol, 22% (v/v) ammonium sulfate, 2.5% (v/v) anthocaynin extract, and pH 3.0) gave 83.7% of extraction yield for anthocyanins. The flavonoids and phenolics are concentrated at the top phase of ATPE with a yield of 78% and 82.5%, respectively. The sugars also partitioned and concentrated at the bottom phase with a yield of 79.5%. The stability studies showed that anthocyanins are very stable between the temperature range of 40−70 °C and the pH range of 3.0−6.0, respectively. At a temperature of 70 °C and at pH 6.0 nearly more than 70% of anthocyanin yield is obtained. The results indicated that ATPE technique have a great promise for the efficient recovery of anthocyanins and also removing of the impurities such as sugars.
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