Serial femtosecond X-ray diffraction of enveloped virus microcrystals

Robert M. Lawrence; Chelsie E. Conrad; Nadia A. Zatsepin; Thomas D. Grant; Haiguang Liu; Daniel James; Garrett Nelson; Ganesh Subramanian; Andrew Aquila; Mark S. Hunter; Mengning Liang; Sébastien Boutet; Jesse Coe; John C. H. Spence; Uwe Weierstall; Wei Liu; Petra Fromme; Vadim Cherezov; Brenda G. Hogue

doi:10.1063/1.4929410

Structural Dynamics (Jul 2015)

Serial femtosecond X-ray diffraction of enveloped virus microcrystals

Robert M. Lawrence,
Chelsie E. Conrad,
Nadia A. Zatsepin,
Thomas D. Grant,
Haiguang Liu,
Daniel James,
Garrett Nelson,
Ganesh Subramanian,
Andrew Aquila,
Mark S. Hunter,
Mengning Liang,
Sébastien Boutet,
Jesse Coe,
John C. H. Spence,
Uwe Weierstall,
Wei Liu,
Petra Fromme,
Vadim Cherezov,
Brenda G. Hogue

Affiliations

Robert M. Lawrence: Biodesign Institute, Arizona State University, Tempe, Arizona 85287, USA
Chelsie E. Conrad: Biodesign Institute, Arizona State University, Tempe, Arizona 85287, USA
Nadia A. Zatsepin: Biodesign Institute, Arizona State University, Tempe, Arizona 85287, USA
Thomas D. Grant: Hauptman-Woodward Institute, State University of New York, Buffalo, New York 14203, USA
Haiguang Liu: Department of Physics, Arizona State University, Tempe, Arizona 85287, USA
Daniel James: Biodesign Institute, Arizona State University, Tempe, Arizona 85287, USA
Garrett Nelson: Biodesign Institute, Arizona State University, Tempe, Arizona 85287, USA
Ganesh Subramanian: Biodesign Institute, Arizona State University, Tempe, Arizona 85287, USA
Andrew Aquila: Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA
Mark S. Hunter: Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA
Mengning Liang: Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA
Sébastien Boutet: Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA
Jesse Coe: Biodesign Institute, Arizona State University, Tempe, Arizona 85287, USA
John C. H. Spence: Biodesign Institute, Arizona State University, Tempe, Arizona 85287, USA
Uwe Weierstall: Biodesign Institute, Arizona State University, Tempe, Arizona 85287, USA
Wei Liu: Biodesign Institute, Arizona State University, Tempe, Arizona 85287, USA
Petra Fromme: Biodesign Institute, Arizona State University, Tempe, Arizona 85287, USA
Vadim Cherezov: Department of Chemistry, Bridge Institute, University of Southern California, Los Angeles, California 90089, USA
Brenda G. Hogue: Biodesign Institute, Arizona State University, Tempe, Arizona 85287, USA

DOI: https://doi.org/10.1063/1.4929410
Journal volume & issue: Vol. 2, no. 4
pp. 041720 – 041720-14

Abstract

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Serial femtosecond crystallography (SFX) using X-ray free-electron lasers has produced high-resolution, room temperature, time-resolved protein structures. We report preliminary SFX of Sindbis virus, an enveloped icosahedral RNA virus with ∼700 Å diameter. Microcrystals delivered in viscous agarose medium diffracted to ∼40 Å resolution. Small-angle diffuse X-ray scattering overlaid Bragg peaks and analysis suggests this results from molecular transforms of individual particles. Viral proteins undergo structural changes during entry and infection, which could, in principle, be studied with SFX. This is an important step toward determining room temperature structures from virus microcrystals that may enable time-resolved studies of enveloped viruses.

Published in Structural Dynamics

ISSN: 2329-7778 (Online)
Publisher: AIP Publishing LLC and ACA
Country of publisher: United States
LCC subjects: Science: Chemistry: Crystallography
Website: http://sd.aip.org

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