Functional distinction of hyphal compartments

Abstract

Abstract Hyphae of higher fungi grow at their tips and are compartmentalized by porous septa that enable inter-compartmental cytoplasmic streaming. Woronin bodies discontinue cytoplasmic streaming by plugging the septal pores. Here, it was assessed whether apical compartments of Aspergillus niger sustain their own growth or whether their growth depends on subapical compartments. Hyphae of wildtype and the ΔhexA strain, lacking Woronin bodies, had a similar morphology and growth rate. A total of 58% and 17% of the hyphae continued growing, respectively, after dissecting the 2nd compartment. Extension rate of the apical compartments that continued growing was not affected, even when the carbon or nitrogen source was limiting. Thus, apical compartments are self-sustaining in growth. It was also shown that the first 8 subapical compartments of the wildtype, but not of the ΔhexA strain, function as a backup system for growth by forming new branches when their apical neighbouring compartment has been damaged. This backup system is pivotal in nature because of the life style of fungi to continuously explore their surrounding substrate that may prove hostile.