A novel chemerin receptor 1 (Chemerin1) takes part in the immune response of cobia (Rachycentron canadum)

Biao Jiang; Caiyi Wen; Yanxin Sun; Wei Li; Chun Liu; Juan Feng; Youlu Su

Fish and Shellfish Immunology Reports (Dec 2022)

A novel chemerin receptor 1 (Chemerin1) takes part in the immune response of cobia (Rachycentron canadum)

Biao Jiang,
Caiyi Wen,
Yanxin Sun,
Wei Li,
Chun Liu,
Juan Feng,
Youlu Su

Affiliations

Biao Jiang: Innovative Institute of Animal Healthy breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510222, Guangdong, China
Caiyi Wen: Innovative Institute of Animal Healthy breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510222, Guangdong, China
Yanxin Sun: Innovative Institute of Animal Healthy breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510222, Guangdong, China
Wei Li: Innovative Institute of Animal Healthy breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510222, Guangdong, China
Chun Liu: Innovative Institute of Animal Healthy breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510222, Guangdong, China
Juan Feng: Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, Guangdong, China
Youlu Su: Innovative Institute of Animal Healthy breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510222, Guangdong, China; Correspondence author.

Journal volume & issue: Vol. 3
p. 100057

Abstract

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Chemerin receptor 1 (Chemerin1) plays a critical role in innate and adaptive immune systems. In this study, a cobia (Rachycentron canadum) Chemerin1 was identified, and its molecular characterization and expression patterns were analyzed. Multiple sequence alignment revealed that the RcChemerin1 possessed a typical dynein regulatory complex (DRC) motif. There was also a potential N-glycosylation site in the extracellular regions of the N-terminus and intracellular loops (ICL) 1 region. Phylogenetic analysis demonstrated that the RcChemerin1 was clustered together with homologous proteins from other fish species. RcChemerin1 was constitutively expressed in a wide range of tissues (especially in immune-related tissues) with different expression levels, which suggests that the RcChemerin1 plays different roles in un-stimulated tissues. RcChemerin1 expression showed up-regulation in the head kidney after Vibrio harveyi challenge. Up-regulation in the head kidney and spleen was also observed after polyinosinic-polycytidylic acid (poly I: C) challenge, which suggests that RcChemerin1 may play vital roles during bacterial and viral infection. The differential responses of immune organs to bacteria and poly I: C imply the differences in defense mechanisms against viruses and bacteria.

Published in Fish and Shellfish Immunology Reports

ISSN: 2667-0119 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Science: Zoology
Website: https://www.journals.elsevier.com/fish-and-shellfish-immunology-reports/

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