Nature Communications (Mar 2019)
Diversifying the structure of zinc finger nucleases for high-precision genome editing
- David E. Paschon,
- Stephanie Lussier,
- Tenzin Wangzor,
- Danny F. Xia,
- Patrick W. Li,
- Sarah J. Hinkley,
- Nicholas A. Scarlott,
- Stephen C. Lam,
- Adam J. Waite,
- Lynn N. Truong,
- Nimisha Gandhi,
- Bhakti N. Kadam,
- Deepak P. Patil,
- David A. Shivak,
- Gary K. Lee,
- Michael C. Holmes,
- Lei Zhang,
- Jeffrey C. Miller,
- Edward J. Rebar
Affiliations
- David E. Paschon
- Sangamo Therapeutics, Inc.
- Stephanie Lussier
- Sangamo Therapeutics, Inc.
- Tenzin Wangzor
- Sangamo Therapeutics, Inc.
- Danny F. Xia
- Sangamo Therapeutics, Inc.
- Patrick W. Li
- Sangamo Therapeutics, Inc.
- Sarah J. Hinkley
- Sangamo Therapeutics, Inc.
- Nicholas A. Scarlott
- Sangamo Therapeutics, Inc.
- Stephen C. Lam
- Sangamo Therapeutics, Inc.
- Adam J. Waite
- Sangamo Therapeutics, Inc.
- Lynn N. Truong
- Sangamo Therapeutics, Inc.
- Nimisha Gandhi
- Sangamo Therapeutics, Inc.
- Bhakti N. Kadam
- Sangamo Therapeutics, Inc.
- Deepak P. Patil
- Sangamo Therapeutics, Inc.
- David A. Shivak
- Sangamo Therapeutics, Inc.
- Gary K. Lee
- Sangamo Therapeutics, Inc.
- Michael C. Holmes
- Sangamo Therapeutics, Inc.
- Lei Zhang
- Sangamo Therapeutics, Inc.
- Jeffrey C. Miller
- Sangamo Therapeutics, Inc.
- Edward J. Rebar
- Sangamo Therapeutics, Inc.
- DOI
- https://doi.org/10.1038/s41467-019-08867-x
- Journal volume & issue
-
Vol. 10,
no. 1
pp. 1 – 12
Abstract
Genome editing often requires cleavage within a narrow sequence window. Here the authors develop an expanded set of zinc finger nuclease architectures that increase the available configurations by a factor of 64 and can target almost every base at loci of therapeutic significance.
