Diversity of Monofloral Honey Based on the Antimicrobial and Antioxidant Potential
Anca Hulea,
Diana Obiștioiu,
Ileana Cocan,
Ersilia Alexa,
Monica Negrea,
Alina-Georgeta Neacșu,
Călin Hulea,
Corina Pascu,
Luminita Costinar,
Ionica Iancu,
Emil Tîrziu,
Viorel Herman
Affiliations
Anca Hulea
Faculty of Veterinary Medicine, Banat University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania” Timisoara, Calea Aradului No. 119, 300645 Timisoara, Romania
Diana Obiștioiu
Faculty of Veterinary Medicine, Banat University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania” Timisoara, Calea Aradului No. 119, 300645 Timisoara, Romania
Ileana Cocan
Faculty of Food Engineering, Banat University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania” Timisoara, Calea Aradului No. 119, 300645 Timisoara, Romania
Ersilia Alexa
Faculty of Food Engineering, Banat University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania” Timisoara, Calea Aradului No. 119, 300645 Timisoara, Romania
Monica Negrea
Faculty of Food Engineering, Banat University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania” Timisoara, Calea Aradului No. 119, 300645 Timisoara, Romania
Alina-Georgeta Neacșu
Faculty of Agriculture, Banat University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania” Timisoara, Calea Aradului No. 119, 300641 Timisoara, Romania
Călin Hulea
Faculty of Veterinary Medicine, Banat University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania” Timisoara, Calea Aradului No. 119, 300645 Timisoara, Romania
Corina Pascu
Faculty of Veterinary Medicine, Banat University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania” Timisoara, Calea Aradului No. 119, 300645 Timisoara, Romania
Luminita Costinar
Faculty of Veterinary Medicine, Banat University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania” Timisoara, Calea Aradului No. 119, 300645 Timisoara, Romania
Ionica Iancu
Faculty of Veterinary Medicine, Banat University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania” Timisoara, Calea Aradului No. 119, 300645 Timisoara, Romania
Emil Tîrziu
Faculty of Veterinary Medicine, Banat University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania” Timisoara, Calea Aradului No. 119, 300645 Timisoara, Romania
Viorel Herman
Faculty of Veterinary Medicine, Banat University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania” Timisoara, Calea Aradului No. 119, 300645 Timisoara, Romania
This study aimed to investigate the antioxidant profile and the antimicrobial activity of four different types of monofloral honey (manuka (MH), brassica rapeseed (BH), acacia (AH), and linden honey (LH)) against some bacterial/fungal ATCC strains and some multidrug-resistant strains isolated from chronic otitis in dogs. For the characterisation of the antioxidant profile of each honey, we extracted the honey samples by hydroalcoholic extraction and analysed them in terms of total polyphenols (TPC), total flavonoids (TFC), and 2,2-diphenyl-1-picrylhydrazyl (DPPH) using the spectrophotometric method. The antimicrobial activity was determined using the microdilution method at concentrations of 10%, 15%, and 20%, with the results expressed in OD (optical density) calculated as BIR% (bacterial inhibition rate)/MIR% (mycelial inhibition rate). The antioxidant characterisation of the analysed honey samples showed the highest antioxidant activity and concentrations of TPC and TFC in MH, followed by LH. MH was proven to be the most effective on most clinical isolates concerning the antimicrobial activity in comparison with BH, AH, and LH. Except for B. cepacia and P. vulgaris, all the clinical isolates were sensitive to the antibacterial activity of honey. Regarding the ATCC strains, MH 10% was the most effective in inhibiting all the strains tested except for P. aeruginosa. In conclusion, the efficacy classification in our study was MH > BH > AH > LH.