Эпидемиология и вакцинопрофилактика (Jun 2016)

Study of Antigenic properties of Human immunodeficiency Virus NEF protein Recombinant Analog

  • G. I. Alatortseva,
  • M. N. Nosik,
  • L. N. Nesterenko,
  • I. I. Amiantova,
  • V. V. Dotsenko,
  • L. N. Luhverchik,
  • M. V. Zhuckina,
  • V. U. Kabargina,
  • E. N. Kudryavtseva,
  • V. V. Zverev

DOI
https://doi.org/10.31631/2073-3046-2016-15-3-83-93
Journal volume & issue
Vol. 15, no. 3
pp. 83 – 93

Abstract

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Protein p27/p25 is a product of HIV nef gene and is a multifunctional factor of HIV pathogenicity. Antigenic properties of recombinant NEF polypeptide which includes N-terminal fragment of HIV-1 p27 protein fused to E. coli ß-galactosidase were studied by serological and virological methods. The interaction was shown between recombinant NEF antigen and serums of HIV-positive individuals by ELISA, Western blot and line immunoassay. There was no interaction with sera of healthy individuals. The antigen specificity of recombinant antigen was shown in the reactions with commercial HIV-1 p27/p25 protein analog and with polyclonal antibodies to synthetic peptides corresponding to N-, C-terminal regions of HIV-1 p27/p25 protein. The interaction between IgG of rabbits immunized with recombinant NEF antigen and viral antigens was shown by indirect immunofluorescence and neutralizing assays. Thus it was proven the possibility of using recombinant NEF protein as an antigen for diagnostic and experimental purposes.

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