PHB3 Maintains Root Stem Cell Niche Identity through ROS-Responsive AP2/ERF Transcription Factors in Arabidopsis
Xiangpei Kong,
Huiyu Tian,
Qianqian Yu,
Feng Zhang,
Rong Wang,
Shan Gao,
Wenhong Xu,
Jiajia Liu,
Eilon Shani,
Chunxiang Fu,
Gongke Zhou,
Liangran Zhang,
Xiansheng Zhang,
Zhaojun Ding
Affiliations
Xiangpei Kong
The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, College of Life Sciences, Shandong University, Jinan, 250100 Shandong, China
Huiyu Tian
The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, College of Life Sciences, Shandong University, Jinan, 250100 Shandong, China
Qianqian Yu
College of Life Sciences, Liaocheng University, Liaocheng, 252000 Shandong, China
Feng Zhang
The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, College of Life Sciences, Shandong University, Jinan, 250100 Shandong, China
Rong Wang
College of Nursing, Xi’an Siyuan University, Xi’an, 710038 Shaanxi, China
Shan Gao
College of Life Sciences, Qilu Normal University, Jinan, 250200 Shandong, China
Wenhong Xu
The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, College of Life Sciences, Shandong University, Jinan, 250100 Shandong, China
Jiajia Liu
The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, College of Life Sciences, Shandong University, Jinan, 250100 Shandong, China
Eilon Shani
Department of Molecular Biology and Ecology of Plants, Tel Aviv University, Tel Aviv 69978, Israel
Chunxiang Fu
Shandong Provincial Key Laboratory of Energy Genetics, Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101 Shandong, China
Gongke Zhou
Shandong Provincial Key Laboratory of Energy Genetics, Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101 Shandong, China
Liangran Zhang
The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, College of Life Sciences, Shandong University, Jinan, 250100 Shandong, China
Xiansheng Zhang
State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai’ an, 271018 Shandong, China
Zhaojun Ding
The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, College of Life Sciences, Shandong University, Jinan, 250100 Shandong, China; Corresponding author
Summary: The root stem cell niche, which is composed of four mitotically inactive quiescent center (QC) cells and the surrounding actively divided stem cells in Arabidopsis, is critical for growth and root development. Here, we demonstrate that the Arabidopsis prohibitin protein PHB3 is required for the maintenance of root stem cell niche identity by both inhibiting proliferative processes in the QC and stimulating cell division in the proximal meristem (PM). PHB3 coordinates cell division and differentiation in the root apical meristem by restricting the spatial expression of ethylene response factor (ERF) transcription factors 115, 114, and 109. ERF115, ERF114, and ERF109 mediate ROS signaling, in a PLT-independent manner, to control root stem cell niche maintenance and root growth through phytosulfokine (PSK) peptide hormones in Arabidopsis. : Prohibitins (PHBs) have been identified as negative regulators of the cell cycle. Here, Kong et al. demonstrate that Arabidopsis PHB3 is essential to maintain QC and DSC identity, and it controls root meristem activity by restricting the spatial expression of the ROS-responsive factors ERF115, 114, and 109 in root. Keywords: root, stem cell niche, prohibitin 3, ROS, ERF, PSK
