Elastin increases biofilm and extracellular matrix production of Aspergillus fumigatus

Brazilian Journal of Microbiology. 49(3):675-682 DOI 10.1016/j.bjm.2017.10.004

 

Journal Homepage

Journal Title: Brazilian Journal of Microbiology

ISSN: 1517-8382 (Print); 1678-4405 (Online)

Publisher: Sociedade Brasileira de Microbiologia

LCC Subject Category: Science: Microbiology

Country of publisher: Brazil

Language of fulltext: English

Full-text formats available: PDF, HTML, XML

 

AUTHORS

Ildnay de Souza Lima Brandão
Heloiza Maria da Silva Oliveira-Moraes
Cristina Maria de Souza Motta
Neiva Tinti de Oliveira
Oliane Maria Correia Magalhães

EDITORIAL INFORMATION

Peer review

Editorial Board

Instructions for authors

Time From Submission to Publication: 39 weeks

 

Abstract | Full Text

Abstract Aspergillus fumigatus is an opportunistic saprobe fungus that accounts for 90% of cases of pulmonary aspergillosis in immunosuppressed patients and is known for its angiotropism. When it reaches the respiratory tract, A. fumigatus interacts with structural components and blood vessels of the lungs, such as elastin. To understand the effect of this structural component, we examined the effect of elastin on the production and development of the biofilm of A. fumigatus. In RPMI containing 10 mg/mL of elastin, a significant increase (absorbance p < 0.0001; dry weight p < 0.0001) in the production of biofilm was observed in comparison to when RPMI was used alone, reaching a maximum growth of 18.8 mg (dry weight) of biofilm in 72 h. In addition, elastin stimulates the production (p = 0.0042) of extracellular matrix (ECM) and decreases (p = 0.005) the hydrophobicity during the development of the biofilm. These results suggest that elastin plays an important role in the growth of A. fumigatus and that it participates in the formation of thick biofilm.