Genetics Selection Evolution (May 2000)

A high-density linkage map of the <it>RN </it>region in pigs

  • Chardon Patrick,
  • Kalm Ernst,
  • Robic Annie,
  • Amarger Valérie,
  • Rey Virginie,
  • Rogel-Gaillard Claire,
  • Reinsch Norbert,
  • Paul Sven,
  • Jeon Jin-Tae,
  • Milan Denis,
  • Looft Christian,
  • Andersson Leif

DOI
https://doi.org/10.1186/1297-9686-32-3-321
Journal volume & issue
Vol. 32, no. 3
pp. 321 – 329

Abstract

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Abstract The porcine RN locus affects muscle glycogen content and meat quality. We previously mapped the RN locus to chromosome 15. This study describes the identification of polymorphisms for four class I and four class II markers located in the RN region. Resource families were genotyped with F-SSCP markers (fluorescent single strand conformation polymorphism) and microsatellite markers. Subsequent multipoint linkage analysis revealed the order FN1-IGFBP5-S1000-S1001-IL8RB-VIL1-RN-Sw936-Sw906. The gene order is identical to the previously reported porcine RH map of the same region. The described map will facilitate positional cloning of the RN gene.

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