Memorias do Instituto Oswaldo Cruz (Sep 2011)

Survey of Leptospira spp in pampas deer (Ozotoceros bezoarticus) in the Pantanal wetlands of the state of Mato Grosso do Sul, Brazil by serology and polymerase chain reaction

  • Anahí Souto Vieira,
  • Grácia Maria Soares Rosinha,
  • Carina Elisei de Oliveira,
  • Silvio Arruda Vasconcellos,
  • Paulo Andre Lima-Borges,
  • Walfrido Moraes Tomás,
  • Guilherme Miranda Mourão,
  • Ana Cristina Reis Lacerda,
  • Cleber Oliveira Soares,
  • Flábio Ribeiro de Araújo,
  • Ubiratan Piovezan,
  • Carlos Andre Zucco,
  • Aiesca Oliveira Pellegrin

DOI
https://doi.org/10.1590/S0074-02762011000600019
Journal volume & issue
Vol. 106, no. 6
pp. 763 – 768

Abstract

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This work reports a survey of Leptospira spp in pampas deer (Ozotoceros bezoarticus) in the Pantanal wetlands of the state of Mato Grosso do Sul, Brazil by serology and polymerase chain reaction (PCR). Seventy pampas deer were captured in the dry season and surveyed using PCR, microscopic agglutination test (MAT) (n = 51) and by both techniques (n = 47). PCR detected infections in two pampas deer and MAT detected infections in three. Through sequencing and phylogenetic analyses, the PCR-amplified fragment detected in deer was identified as Leptospira interrogans. Serovars Pomona and Butembo were detected using MAT and the highest titre was 200 for serovar Pomona. Epidemiological aspects of the findings are discussed.

Keywords