Protective Effect of Quercetin against Lower Extremity Ischaemia-Reperfusion Damage in Rats and investigation of TAS-TOS levels

Abstract

INTRODUCTION: It was aimed to determine the protective effectiveness of quercetin in preventing lower extremity ischemia-reperfusion (I/R) injury. METHODS: 30 Sprague-Dawley male rats weighing 250-350 g were divided into three groups. Anaesthesia was applied to the first group of rats without ischemia-reperfusion process. To the second group two hours of ischemia and two hours of reperfusion, with the help of left lower extremities tourniquet, were applied. To the third group, 50mg/kg (ip) quercetin application was performed 45 minutes before the ischemia was initiated, two hours of ischemia and two hours of reperfusion application were carried out. At the end of the process, by taking the tissue samples, total antioxidant status (TAS) and total oxidant status (TOS) levels were evaluated. RESULTS: When TAS was evaluated, it was determined that the decrease (p˂0.001) occurring in I/R group in comparison with the control group was significant, the decrease (p˂0.05) in I/R+Q group in comparison with control group, and again the increase (p˂0.001) in I/R+Q group in comparison with I/R group was also significant. When the TOS amounts were evaluated, it was determined that the increase (p˂0.001) occurring in I/R group in comparison with control group, the increase in I/R+Q group in comparison with control group (p˂0.05), the decrease in I/R+Q group in comparison with I/R group was statistically significant. DISCUSSION AND CONCLUSION: It has been determined that quercetin has protective effect and decrease the injury occurred in experimental Lower Extremity Ischemia- Reperfusion Injury in Rats.

Keywords

• ischemia / reperfusion injury
• quercetin
• lower extremity.