Genome-Wide Analysis and Expression Profiling of <i>DUF668</i> Genes in <i>Glycine max</i> under Salt Stress
Madiha Zaynab,
Yasir Sharif,
Zhaoshi Xu,
Sajid Fiaz,
Rashid Al-Yahyai,
Hamad. A. Yadikar,
Najla Amin T. Al Kashgry,
Sameer H. Qari,
Monther Sadder,
Shuangfei Li
Affiliations
Madiha Zaynab
College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China
Yasir Sharif
College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Zhaoshi Xu
Institute of Crop Science, Chinese Academy of Agricultural Sciences, National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Beijing 100081, China
Sajid Fiaz
Department of Plant Breeding and Genetics, The University of Haripur, Haripur 22620, Pakistan
Rashid Al-Yahyai
Department of Plant Sciences, College of Agricultural and Marine Sciences, Sultan Qaboos University, Al-Khod, P.O. Box 34, Muscat 123, Oman
Hamad. A. Yadikar
Department of Biological Sciences, Faculty of Science, Kuwait University, P.O. Box 5969, Safat 13060, Kuwait
Najla Amin T. Al Kashgry
Department of Biology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia
Sameer H. Qari
Department of Biology, Al-Jumum University College, Umm Al-Qura University, Makkah 21955, Saudi Arabia
Monther Sadder
School of Agriculture, University of Jordan, Amman 11942, Jordan
Shuangfei Li
Shenzhen Key Laboratory of Marine Bioresource & Eco-Environmental Sciences, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China
The DUF668 gene performs a critical role in mitigating the impact of abiotic stress factors. In this study, we identified 30 DUF668 genes in a soybean genome, distributed across fifteen chromosomes. The phylogenetic analysis classified the DUF668 genes into three groups (group I, group II, and group III). Interestingly, gene structure analysis illustrated that several GmDUF668 genes were without introns. Furthermore, the subcellular localization results suggested that GmDUF668 proteins were present in the nucleus, mitochondria, cytoplasm, and plasma membrane. GmDUF668 promoters were analyzed in silico to gain insight into the presence of regulatory sequences for TFs binding. The expression profiling illustrated that GmDUF668 genes showed expression in leaves, roots, nodules, and flowers. To investigate their response to salt stress, we utilized the RNA sequencing data of GmDUF668 genes. The results unveiled that GmDUF668-8, GmDUF668-20, and GmDUF668-30 genes were upregulated against salt stress treatment. We further validated these findings using qRT-PCR analysis. These findings provide a scientific basis to explore the functions of GmDUF668 genes against different stress conditions.
