Novel Antimicrobial Agents Based on Zinc-Doped Hydroxyapatite Loaded with Tetracycline
Simona Liliana Iconaru,
Daniela Predoi,
Carmen Steluta Ciobanu,
Catalin Constantin Negrila,
Roxana Trusca,
Steinar Raaen,
Krzysztof Rokosz,
Liliana Ghegoiu,
Monica Luminita Badea,
Carmen Cimpeanu
Affiliations
Simona Liliana Iconaru
National Institute of Materials Physics, Atomistilor Street, No. 405A, 077125 Magurele, Romania
Daniela Predoi
National Institute of Materials Physics, Atomistilor Street, No. 405A, 077125 Magurele, Romania
Carmen Steluta Ciobanu
National Institute of Materials Physics, Atomistilor Street, No. 405A, 077125 Magurele, Romania
Catalin Constantin Negrila
National Institute of Materials Physics, Atomistilor Street, No. 405A, 077125 Magurele, Romania
Roxana Trusca
National Centre for Micro and Nanomaterials, University Politehnica of Bucharest, 060042 Bucharest, Romania
Steinar Raaen
Department of Physics, Norwegian University of Science and Technology (NTNU), Realfagbygget E3-124 Høgskoleringen 5, NO 7491 Trondheim, Norway
Krzysztof Rokosz
Faculty of Electronics and Computer Science, Koszalin University of Technology, Śniadeckich 2, PL 75-453 Koszalin, Poland
Liliana Ghegoiu
National Institute of Materials Physics, Atomistilor Street, No. 405A, 077125 Magurele, Romania
Monica Luminita Badea
Faculty of Horticulture, University of Agronomic Sciences and Veterinary Medicine, 59 Marasti Boulevard, 011464 Bucharest, Romania
Carmen Cimpeanu
Faculty of Land Reclamation and Environmental Engineering, University of Agronomic Sciences and Veterinary Medicine of Bucharest, 59 Marasti Boulevard, 011464 Bucharest, Romania
In this paper, we present for the first time the development of zinc-doped hydroxyapatite enriched with tetracycline (ZnHApTe) powders and provide a comprehensive evaluation of their physico-chemical and biological properties. Various techniques such as X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), and Fourier transform infrared spectroscopy (FTIR) were used for the sample’s complex evaluation. Moreover, the biocompatibility of zinc-doped hydroxyapatite (ZnHAp) and ZnHApTe nanoparticles was evaluated with the aid of human fetal osteoblastic cells (hFOB 1.19 cell line). The results of the biological assays suggested that these nanoparticles hold great promise as potential candidates for the future development of novel biocompatible and antimicrobial agents for biomedical applications. The antimicrobial properties of the ZnHAp and ZnHApTe nanoparticles were assessed using the standard reference microbial strains Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, and Candida albicans ATCC 10231. The results of the in vitro antimicrobial assay demonstrated that both tested materials exhibited good antimicrobial activity. Additionally, these data also indicated that the antimicrobial effects of the ZnHAp nanoparticles were intensified by the presence of tetracycline (Te). Furthermore, the results also suggested that the antimicrobial activity of the samples increased with the incubation time.
