Cord Blood Plasma and Placental Mesenchymal Stem Cells-Derived Exosomes Increase Ex Vivo Expansion of Human Cord Blood Hematopoietic Stem Cells While Maintaining Their Stemness
Rasha S. Teleb,
Amal Abdul-Hafez,
Amira Othman,
Ahmed El-Abd Ahmed,
Abdelrahman A. Elsaid,
Hattan Arif,
Ahmed A. Zarea,
Mohammed Abdulmageed,
Hend Mohamed,
Sherif Abdelfattah Ibrahim,
Ranga P. Thiruvenkataramani,
Tarek Mohamed,
Masamitsu Kanada,
Burra V. Madhukar,
Myrna Gonzalez Arellano,
Mohammed M. Sayed,
Heba M. Qubaisy,
Said A. Omar
Affiliations
Rasha S. Teleb
Division of Neonatology, Department of Pediatrics and Human Development, College of Human Medicine, Michigan State University, East Lansing, MI 48824, USA
Amal Abdul-Hafez
Division of Neonatology, Department of Pediatrics and Human Development, College of Human Medicine, Michigan State University, East Lansing, MI 48824, USA
Amira Othman
Division of Neonatology, Department of Pediatrics and Human Development, College of Human Medicine, Michigan State University, East Lansing, MI 48824, USA
Ahmed El-Abd Ahmed
Department of Pediatrics and Neonatology, Faculty of Medicine, South Valley University, Qena 83523, Egypt
Abdelrahman A. Elsaid
Clinical Pathology Department, Faculty of Medicine, South Valley University, Qena 83523, Egypt
Hattan Arif
Division of Neonatology, Department of Pediatrics and Human Development, College of Human Medicine, Michigan State University, East Lansing, MI 48824, USA
Ahmed A. Zarea
The Institute for Quantitative Health Science & Engineering, College of Human Medicine, Michigan State University, East Lansing, MI 48824, USA
Mohammed Abdulmageed
Division of Neonatology, Department of Pediatrics and Human Development, College of Human Medicine, Michigan State University, East Lansing, MI 48824, USA
Hend Mohamed
Division of Neonatology, Department of Pediatrics and Human Development, College of Human Medicine, Michigan State University, East Lansing, MI 48824, USA
Sherif Abdelfattah Ibrahim
Division of Neonatology, Department of Pediatrics and Human Development, College of Human Medicine, Michigan State University, East Lansing, MI 48824, USA
Ranga P. Thiruvenkataramani
Division of Neonatology, Department of Pediatrics and Human Development, College of Human Medicine, Michigan State University, East Lansing, MI 48824, USA
Tarek Mohamed
Division of Neonatology, Department of Pediatrics and Human Development, College of Human Medicine, Michigan State University, East Lansing, MI 48824, USA
Masamitsu Kanada
The Institute for Quantitative Health Science & Engineering, College of Human Medicine, Michigan State University, East Lansing, MI 48824, USA
Burra V. Madhukar
Division of Neonatology, Department of Pediatrics and Human Development, College of Human Medicine, Michigan State University, East Lansing, MI 48824, USA
Myrna Gonzalez Arellano
Division of Neonatology, Department of Pediatrics and Human Development, College of Human Medicine, Michigan State University, East Lansing, MI 48824, USA
Mohammed M. Sayed
Department of Pediatrics and Neonatology, Faculty of Medicine, South Valley University, Qena 83523, Egypt
Heba M. Qubaisy
Department of Pediatrics and Neonatology, Faculty of Medicine, South Valley University, Qena 83523, Egypt
Said A. Omar
Division of Neonatology, Department of Pediatrics and Human Development, College of Human Medicine, Michigan State University, East Lansing, MI 48824, USA
Background: Mesenchymal stem cells (MSCs) have been used for ex vivo expansion of umbilical cord blood (UCB) hematopoietic stem cells (HSCs) to maintain their primitive characters and long-term reconstitution abilities during transplantation. Therapeutic effects of MSCs mainly rely on paracrine mechanisms, including secretion of exosomes (Exos). The objective of this study was to examine the effect of cord blood plasma (CBP)-derived Exos (CBP Exos) and Placental MSCs-derived Exos (MSCs Exos) on the expansion of UCB HSCs to increase their numbers and keep their primitive characteristics. Methods: CD34+ cells were isolated from UCB, cultured for 10 days, and the expanded HSCs were sub-cultured in semisolid methylcellulose media for primitive colony forming units (CFUs) assay. MSCs were cultured from placental chorionic plates. Results: CBP Exos and MSCs Exos compared with the control group significantly increased the number of total nucleated cells (TNCs), invitro expansion of CD34+ cells, primitive subpopulations of CD34+38+ and CD34+38−Lin− cells (p p < 0.01). Conclusion: CBP- and placental-derived exosomes are associated with significant ex vivo expansion of UCB HSCs, while maintaining their primitive characters and may eliminate the need for transplantation of an additional unit of UCB.
