Molecular mechanics underlying flat-to-round membrane budding in live secretory cells

Wonchul Shin; Ben Zucker; Nidhi Kundu; Sung Hoon Lee; Bo Shi; Chung Yu Chan; Xiaoli Guo; Jonathan T. Harrison; Jaymie Moore Turechek; Jenny E. Hinshaw; Michael M. Kozlov; Ling-Gang Wu

doi:10.1038/s41467-022-31286-4

Nature Communications (Jun 2022)

Molecular mechanics underlying flat-to-round membrane budding in live secretory cells

Wonchul Shin,
Ben Zucker,
Nidhi Kundu,
Sung Hoon Lee,
Bo Shi,
Chung Yu Chan,
Xiaoli Guo,
Jonathan T. Harrison,
Jaymie Moore Turechek,
Jenny E. Hinshaw,
Michael M. Kozlov,
Ling-Gang Wu

Affiliations

Wonchul Shin: National Institute of Neurological Disorders and Stroke
Ben Zucker: Department of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel Aviv University
Nidhi Kundu: Structural Cell Biology Section, Laboratory of Cell and Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases
Sung Hoon Lee: Chung-Ang University
Bo Shi: National Institute of Neurological Disorders and Stroke
Chung Yu Chan: National Institute of Neurological Disorders and Stroke
Xiaoli Guo: National Institute of Neurological Disorders and Stroke
Jonathan T. Harrison: Structural Cell Biology Section, Laboratory of Cell and Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases
Jaymie Moore Turechek: National Institute of Neurological Disorders and Stroke
Jenny E. Hinshaw: Structural Cell Biology Section, Laboratory of Cell and Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases
Michael M. Kozlov: Department of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel Aviv University
Ling-Gang Wu: National Institute of Neurological Disorders and Stroke

DOI: https://doi.org/10.1038/s41467-022-31286-4
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 18

Abstract

Read online

Shin et al. report the molecular mechanics of membrane budding: actin and dynamin pull membrane inward to form a Λ-shape profile; dynamin helices convert Λ- to Ω-shape by constricting Λ’s base, and then constrict Ω-profile’s pore to form a vesicle.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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