Pain- and morphine-associated transcriptional regulation of neuropeptide FF and the G-protein-coupled NPFF2 receptor gene

Johanna M Nystedt; Kim Lemberg; Minnamaija Lintunen; Kaarin Mustonen; Reetta Holma; Vesa K Kontinen; Eija Kalso; Pertti Panula

Neurobiology of Disease (Jun 2004)

Pain- and morphine-associated transcriptional regulation of neuropeptide FF and the G-protein-coupled NPFF2 receptor gene

Johanna M Nystedt,
Kim Lemberg,
Minnamaija Lintunen,
Kaarin Mustonen,
Reetta Holma,
Vesa K Kontinen,
Eija Kalso,
Pertti Panula

Affiliations

Johanna M Nystedt: Department of Biology, Åbo Akademi University, 20520 Turku, Finland; Neuroscience Center and Institute of Biomedicine/Anatomy, University of Helsinki, 00290 Helsinki, Finland; Instititute of Biomedicine/Pharmacology, University of Helsinki, 00290 Helsinki, Finland; Pain clinic, Department of Anaesthesia and Intensive Care Medicine, Helsinki University Hospital, 00290 Helsinki, Finland
Kim Lemberg: Department of Biology, Åbo Akademi University, 20520 Turku, Finland; Neuroscience Center and Institute of Biomedicine/Anatomy, University of Helsinki, 00290 Helsinki, Finland; Instititute of Biomedicine/Pharmacology, University of Helsinki, 00290 Helsinki, Finland; Pain clinic, Department of Anaesthesia and Intensive Care Medicine, Helsinki University Hospital, 00290 Helsinki, Finland
Minnamaija Lintunen: Department of Biology, Åbo Akademi University, 20520 Turku, Finland; Neuroscience Center and Institute of Biomedicine/Anatomy, University of Helsinki, 00290 Helsinki, Finland; Instititute of Biomedicine/Pharmacology, University of Helsinki, 00290 Helsinki, Finland; Pain clinic, Department of Anaesthesia and Intensive Care Medicine, Helsinki University Hospital, 00290 Helsinki, Finland
Kaarin Mustonen: Department of Biology, Åbo Akademi University, 20520 Turku, Finland; Neuroscience Center and Institute of Biomedicine/Anatomy, University of Helsinki, 00290 Helsinki, Finland; Instititute of Biomedicine/Pharmacology, University of Helsinki, 00290 Helsinki, Finland; Pain clinic, Department of Anaesthesia and Intensive Care Medicine, Helsinki University Hospital, 00290 Helsinki, Finland
Reetta Holma: Department of Biology, Åbo Akademi University, 20520 Turku, Finland; Neuroscience Center and Institute of Biomedicine/Anatomy, University of Helsinki, 00290 Helsinki, Finland; Instititute of Biomedicine/Pharmacology, University of Helsinki, 00290 Helsinki, Finland; Pain clinic, Department of Anaesthesia and Intensive Care Medicine, Helsinki University Hospital, 00290 Helsinki, Finland
Vesa K Kontinen: Department of Biology, Åbo Akademi University, 20520 Turku, Finland; Neuroscience Center and Institute of Biomedicine/Anatomy, University of Helsinki, 00290 Helsinki, Finland; Instititute of Biomedicine/Pharmacology, University of Helsinki, 00290 Helsinki, Finland; Pain clinic, Department of Anaesthesia and Intensive Care Medicine, Helsinki University Hospital, 00290 Helsinki, Finland
Eija Kalso: Department of Biology, Åbo Akademi University, 20520 Turku, Finland; Neuroscience Center and Institute of Biomedicine/Anatomy, University of Helsinki, 00290 Helsinki, Finland; Instititute of Biomedicine/Pharmacology, University of Helsinki, 00290 Helsinki, Finland; Pain clinic, Department of Anaesthesia and Intensive Care Medicine, Helsinki University Hospital, 00290 Helsinki, Finland
Pertti Panula: Department of Biology, Åbo Akademi University, 20520 Turku, Finland; Neuroscience Center and Institute of Biomedicine/Anatomy, University of Helsinki, 00290 Helsinki, Finland; Instititute of Biomedicine/Pharmacology, University of Helsinki, 00290 Helsinki, Finland; Pain clinic, Department of Anaesthesia and Intensive Care Medicine, Helsinki University Hospital, 00290 Helsinki, Finland

Journal volume & issue: Vol. 16, no. 1
pp. 254 – 262

Abstract

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Neuropeptide FF (NPFF) is involved in pain modulation, especially plasticity during inflammatory and neuropathic pain, and opiate interactions. Its nociceptive functions may be mediated by the NPFF2 receptor. To elucidate the role of the NPFF system in plasticity associated with pathologic pain, we studied the changes of NPFF mRNA and NPFF2 receptor mRNA in rat models of acute colonic inflammation, inflammatory pain, and neuropathic pain. Furthermore, we studied the mRNA levels of both NPFF and NPFF2 receptor in morphine-tolerant rats and after acute morphine injections. We found an activation of spinal NPFF and NPFF2 receptor during early inflammatory pain. Supraspinally, we found an up-regulation of NPFF2 receptor mRNA during acute colonic inflammation and neuropathic pain. Acute, but not chronic, morphine activated the genes supraspinally. The results give further evidence for the involvement of the NPFF system in pain modulation and may provide new therapeutic opportunities for pathologic pain.

Published in Neurobiology of Disease

ISSN: 0969-9961 (Print); 1095-953X (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Medicine: Internal medicine: Neurosciences. Biological psychiatry. Neuropsychiatry
Website: https://www.journals.elsevier.com/neurobiology-of-disease

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