Chemical composition of fixed oil and in vitro antimicrobial activity of Andrographis paniculata root

Abstract

The present study estimates the antimicrobial potential of root extract of Andrographis paniculata which are extensively used in traditional medicine against clinical pathogens. The in vitro antibacterial activities were done with ten pathogen bacterial strains. Among the three extracts, the methanol extract exhibited the highest zone of inhibition against Salmonella typhimurium (45 mm), while ethanol and ethyl acetate extract revealed strong in vitro antibacterial against Shigella flagella (42 mm) and E. faecalis (38 mm), respectively at 200 µg/ml concentration. Antifungal activities of ethanol and methanol extract were done against four reference fungal strains. The ethanol and methanol extract confirmed the highest antifungal activity against Trichophyton mentagrophytes (38 mm) and Candida albicans (38 mm), respectively. The constituents of the fixed oil of petroleum ether root extract were evaluated by Gas Chromatography-Mass Spectrophotometer. Eleven composites have recognized, methyl palmitate (56.732%) was the major constituent. The investigation come to an end that the phytochemical constituents of methanol and ethanol root extract have capacity to kill both drug resistant bacteria and fungal.

Keywords

• Andrographis paniculata
• Antimicrobial activity
• Fixed Oil
• Gas Chromatography–Mass Spectrophotometer