A New Water-Soluble Thermosensitive Star-Like Copolymer as a Promising Carrier of the Chemotherapeutic Drug Doxorubicin
Mariia Chernykh,
Dmytro Zavalny,
Viktoriya Sokolova,
Stanislav Ponomarenko,
Svitlana Prylutska,
Yuliia Kuziv,
Vasyl Chumachenko,
Andrii Marynin,
Nataliya Kutsevol,
Matthias Epple,
Uwe Ritter,
Jacek Piosik,
Yuriy Prylutskyy
Affiliations
Mariia Chernykh
Taras Shevchenko National University of Kyiv, Volodymyrska Street, 64, 01601 Kyiv, Ukraine
Dmytro Zavalny
Taras Shevchenko National University of Kyiv, Volodymyrska Street, 64, 01601 Kyiv, Ukraine
Viktoriya Sokolova
Center for Nanointegration Duisburg-Essen (CeNIDE), Institute of Inorganic Chemistry, University of Duisburg-Essen, University Street, 5-7, 45117 Essen, Germany
Stanislav Ponomarenko
Taras Shevchenko National University of Kyiv, Volodymyrska Street, 64, 01601 Kyiv, Ukraine
Svitlana Prylutska
Department of Physiology, Plant Biochemistry and Bioenergetics, National University of Life and Environmental Science of Ukraine, Heroiv Oborony Street, 15, 03041 Kyiv, Ukraine
Yuliia Kuziv
Taras Shevchenko National University of Kyiv, Volodymyrska Street, 64, 01601 Kyiv, Ukraine
Vasyl Chumachenko
Taras Shevchenko National University of Kyiv, Volodymyrska Street, 64, 01601 Kyiv, Ukraine
Andrii Marynin
National University of Food Technologies of Ukraine, Volodymyrska Street, 01033 Kyiv, Ukraine
Nataliya Kutsevol
Taras Shevchenko National University of Kyiv, Volodymyrska Street, 64, 01601 Kyiv, Ukraine
Matthias Epple
Center for Nanointegration Duisburg-Essen (CeNIDE), Institute of Inorganic Chemistry, University of Duisburg-Essen, University Street, 5-7, 45117 Essen, Germany
Uwe Ritter
Institute of Chemistry and Biotechnology, Technical University of Ilmenau, Weimarer Street, 25, 98693 Ilmenau, Germany
Jacek Piosik
Laboratory of Biophysics, Intercollegiate Faculty of Biotechnology UG-MUG, University of Gdansk, Abrahama 58, 80-307 Gdańsk, Poland
Yuriy Prylutskyy
Taras Shevchenko National University of Kyiv, Volodymyrska Street, 64, 01601 Kyiv, Ukraine
A new water-soluble thermosensitive star-like copolymer, dextran-graft-poly-N-iso-propilacrylamide (D-g-PNIPAM), was created and characterized by various techniques (size-exclusion chromatography, differential scanning calorimetry, Fourier-transform infrared (FTIR) spectroscopy, and dynamic light scattering (DLS) spectroscopy). The viability of cancer cell lines (human transformed cervix epithelial cells, HeLa) as a model for cancer cells was studied using MTT and Live/Dead assays after incubation with a D-g-PNIPAM copolymer as a carrier for the drug doxorubicin (Dox) as well as a D-g-PNIPAM + Dox mixture as a function of the concentration. FTIR spectroscopy clearly indicated the complex formation of Dox with the D-g-PNIPAM copolymer. The size distribution of particles in Hank’s solution was determined by the DLS technique at different temperatures. The in vitro uptake of the studied D-g-PNIPAM + Dox nanoparticles into cancer cells was demonstrated by confocal laser scanning microscopy. It was found that D-g-PNIPAM + Dox nanoparticles in contrast to Dox alone showed higher toxicity toward cancer cells. All of the aforementioned facts indicate a possibility of further preclinical studies of the water-soluble D-g-PNIPAM particles’ behavior in animal tumor models in vivo as promising carriers of anticancer agents.
