Fine mapping of powdery mildew resistance gene PmTm4 in wheat using comparative genomics

Jing-zhong XIE; Li-li WANG; Yong WANG; Huai-zhi ZHANG; Sheng-hui ZHOU; Qiu-hong WU; Yong-xing CHEN; Zhen-zhong WANG; Guo-xin WANG; De-yun ZHANG; Yan ZHANG; Tie-zhu HU; Zhi-yong LIU

Journal of Integrative Agriculture (Mar 2017)

Fine mapping of powdery mildew resistance gene PmTm4 in wheat using comparative genomics

Jing-zhong XIE,
Li-li WANG,
Yong WANG,
Huai-zhi ZHANG,
Sheng-hui ZHOU,
Qiu-hong WU,
Yong-xing CHEN,
Zhen-zhong WANG,
Guo-xin WANG,
De-yun ZHANG,
Yan ZHANG,
Tie-zhu HU,
Zhi-yong LIU

Affiliations

Jing-zhong XIE: State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100193, P.R. China
Li-li WANG: State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100193, P.R. China
Yong WANG: State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100193, P.R. China
Huai-zhi ZHANG: State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100193, P.R. China
Sheng-hui ZHOU: State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100193, P.R. China
Qiu-hong WU: Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, P.R. China
Yong-xing CHEN: State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100193, P.R. China
Zhen-zhong WANG: China Rural Technology Development Center, Beijing 100045, P.R. China
Guo-xin WANG: State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100193, P.R. China
De-yun ZHANG: State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100193, P.R. China
Yan ZHANG: State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100193, P.R. China
Tie-zhu HU: College of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang 453003, P.R. China
Zhi-yong LIU: State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100193, P.R. China; Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, P.R. China; Correspondence LIU Zhi-yong Tel: +86-10-64806422 HU Tie-zhu Tel: +86-373-3040964

Journal volume & issue: Vol. 16, no. 3
pp. 540 – 550

Abstract

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Powdery mildew, caused by Blumeria graminis f. sp. tritici, is one of the most severe wheat diseases. Mining powdery mildew resistance genes in wheat cultivars and their appliance in breeding program is a promising way to control this disease. Genetic analysis revealed that a single dominant resistance gene named PmTm4 originated from Chinese wheat line Tangmai 4 confers resistance to prevailing isolates of B. graminis f. sp. tritici isolate E09. Detailed comparative genomics analyses helped to develop closely linked markers to PmTm4 and a fine genetic map was constructed using large F2 population, in which PmTm4 was located into a 0.66-cM genetic interval. The orthologous subgenome region of PmTm4 in Aegilops tauschii was identified, and two resistance gene analogs (RGA) were characterized from the corresponding sequence scaffolds of Ae. tauschii draft assembly. The closely linked markers and identified Ae. tauschii orthologs in the mapping interval provide an entry point for chromosome landing and map-based cloning of PmTm4.

Published in Journal of Integrative Agriculture

ISSN: 2095-3119 (Print); 2352-3425 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Agriculture: Agriculture (General)
Website: https://www.journals.elsevier.com/journal-of-integrative-agriculture

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