Brazilian Journal of Oral Sciences (Nov 2015)

Polymorphisms in the IL-1a and IL-1b genes are not associated with susceptibility to chronic periodontitis in a brazilian population

  • Paula C. Trevilatto,
  • Raquel M. Scarel-Caminaga,
  • Rui B. Brito Junior,
  • Ana P. Souza,
  • Antonio W. Sallum,
  • Sergio R. P. Line

DOI
https://doi.org/10.20396/bjos.v2i7.8641716
Journal volume & issue
Vol. 2, no. 7

Abstract

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The aim of this study was to investigate the association between polymorphisms in the IL-1a and IL-1b genes and severity of chronic periodontitis in Brazilians. 113 non-smoking subjects over 25 years (mean age 41.6) were divided according to the severity level of periodontal disease: 44 healthy individuals (control group), 31 subjects with moderate and 38 with severe periodontitis. DNA was obtained from epithelial cells through a mouthwash with 3 % glucose and scraping of oral mucosa. The samples were analyzed for IL-1a (- 889) and IL-1b (+3953) polymorphisms using PCR-RFLP. The significance of the differences in the frequencies of the polymorphisms in the control and groups with periodontitis was assessed by Chisquare test (p<0.05). No significant differences in the allele and genotype frequencies were found among the groups. It was concluded that neither polymorphisms IL-1a (-889) and IL-1b (+3953) nor the composite genotype were associated with the severity of chronic periodontal disease in the study population.

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