Multifunctional electrospun nanofibrous scaffold enriched with alendronate and hydroxyapatite for balancing osteogenic and osteoclast activity to promote bone regeneration

Shabnam Anjum; Shabnam Anjum; Dilip Kumar Arya; Mohammad Saeed; Daoud Ali; Mohammad Saud Athar; Wang Yulin; Saud Alarifi; Xixi Wu; P.S. Rajinikanth; Qiang Ao; Qiang Ao

doi:10.3389/fbioe.2023.1302594

Frontiers in Bioengineering and Biotechnology (Nov 2023)

Multifunctional electrospun nanofibrous scaffold enriched with alendronate and hydroxyapatite for balancing osteogenic and osteoclast activity to promote bone regeneration

Shabnam Anjum,
Shabnam Anjum,
Dilip Kumar Arya,
Mohammad Saeed,
Daoud Ali,
Mohammad Saud Athar,
Wang Yulin,
Saud Alarifi,
Xixi Wu,
P.S. Rajinikanth,
Qiang Ao,
Qiang Ao

Affiliations

Shabnam Anjum: Department of Tissue Engineering, School of Intelligent Medicine, China Medical University, Shenyang, Liaoning, China
Shabnam Anjum: NMPA Key Laboratory for Quality Research and Control of Tissue Regenerative Biomaterial, Institute of Regulatory Science for Medical Device, National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, Sichuan, China
Dilip Kumar Arya: Department of Pharmaceutical Sciences, Babasaheb Bhimrao Ambedkar University, Lucknow, India
Mohammad Saeed: Department of Pharmacology, Dr. A.P.J. Abdul Kalam Technical University, Lucknow, India
Daoud Ali: Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia
Mohammad Saud Athar: Department of Chemistry, Aligarh Muslim University, Aligarh, India
Wang Yulin: NMPA Key Laboratory for Quality Research and Control of Tissue Regenerative Biomaterial, Institute of Regulatory Science for Medical Device, National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, Sichuan, China
Saud Alarifi: Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia
Xixi Wu: NMPA Key Laboratory for Quality Research and Control of Tissue Regenerative Biomaterial, Institute of Regulatory Science for Medical Device, National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, Sichuan, China
P.S. Rajinikanth: Department of Pharmaceutical Sciences, Babasaheb Bhimrao Ambedkar University, Lucknow, India
Qiang Ao: Department of Tissue Engineering, School of Intelligent Medicine, China Medical University, Shenyang, Liaoning, China
Qiang Ao: NMPA Key Laboratory for Quality Research and Control of Tissue Regenerative Biomaterial, Institute of Regulatory Science for Medical Device, National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, Sichuan, China

DOI: https://doi.org/10.3389/fbioe.2023.1302594
Journal volume & issue: Vol. 11

Abstract

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Electrospun composite nanofiber scaffolds are well known for their bone and tissue regeneration applications. This research is focused on the development of PVP and PVA nanofiber composite scaffolds enriched with hydroxyapatite (HA) nanoparticles and alendronate (ALN) using the electrospinning technique. The developed nanofiber scaffolds were investigated for their physicochemical as well as bone regeneration potential. The results obtained from particle size, zeta potential, SEM and EDX analysis of HA nanoparticles confirmed their successful fabrication. Further, SEM analysis verified nanofiber’s diameters within 200–250 nm, while EDX analysis confirmed the successful incorporation of HA and ALN into the scaffolds. XRD and TGA analysis revealed the amorphous and thermally stable nature of the nanofiber composite scaffolds. Contact angle, FTIR analysis, Swelling and biodegradability studies revealed the hydrophilicity, chemical compatibility, suitable water uptake capacity and increased in-vitro degradation making it appropriate for tissue regeneration. The addition of HA into nanofiber scaffolds enhanced the physiochemical properties. Additionally, hemolysis cell viability, cell adhesion and proliferation by SEM as well as confocal microscopy and live/dead assay results demonstrated the non-toxic and biocompatibility behavior of nanofiber scaffolds. Alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase (TRAP) assays demonstrated osteoblast promotion and osteoclast inhibition, respectively. These findings suggest that developed HA and ALN-loaded PVP/PVA-ALN-HA nanofiber composite scaffolds hold significant promise for bone regeneration applications.

Published in Frontiers in Bioengineering and Biotechnology

ISSN: 2296-4185 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Technology: Chemical technology: Biotechnology
Website: http://www.frontiersin.org/bioengineering_and_biotechnology

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