Quantum-Mechanical Sensitive Na/Ca Exchange as a Target for Pain Relief Effects of 4Hz Weak

Abstract

A recent study has shown 40Ca and 45Ca leading to activation of RNa/40Ca and RNa/45Ca exchange have pain and pain relief effects, respectively, because of E45>E40 the increase [45Ca]i brings to activation Ca-Calmodulin-NO-cGMP -induced stimulation of Na/Ca exchange in forwarding (F) mode. It was shown also that 4Hz mechanical vibration (MV) has pain relief effect by activation cGMP-dependent (F) Na/Ca exchange however the nature of targets of thermodynamic (E45>E40) and quantum-mechanical stimulation of cGMP-dependent (F) Na/Ca exchange is not clear yet. Therefore it was suggested that the comparative study of 45Ca PS injection and 4Hz MV on thermal pain thresholds (LPPS), tissues hydration and 45Ca uptake at Na/K pump active and inactive states as well as at activation of cAMP-dependent Na/Ca exchange in reverse (R) mode will be possible to evaluate the nature of target through which the pain relief effect of 4Hz MV is realized. Obtained data indicate that the 4Hz MV decrease LPPS in both 40CaPS and 45CaPS injected animals which were accompanied by tissues dehydration and hydrations, compared those in sham animals, as well as the depression of 45Ca uptake by heart and brain cortex, are due to different mechanism than pain relief of 45CaPS effects which accompanied by tissues dehydrations compared with 40CaPS injected animal. The obtained data bring us to the suggestion that the pain relief of both 45CaPS and the 4Hz MV-induced pain relief effects are realized by activation of cGMP-dependent (F) Na/Ca exchange. However the latter takes place in 45CaPS injected animals by activation Ca-Calmoduline-NO-cGMP pathway because of E45> E40-induced higher rate cAMP-dependent (R)Na/Ca exchange while in case of the impact of 4HzMV by direct activation cGMP forming process. Therefore, to avoid the multisided poisoning effects of high [Ca]i, we recommend for pain relief effect to use quantum-mechanical method stimulation of forming cGMP, such as 4Hz MV, instead, factors stimulating high [Ca]i–induced activation of calmodulin-dependent stimulation of cGMP-dependent (F) Na/Ca exchange.

Keywords

• mechanical vibration
• pain relief