BIO Web of Conferences (Jan 2021)

Evaluation of the effectiveness of potato improvement in in vitro culture on optimized nutrient media

  • Gneusheva I.A.,
  • Gavrilova A.Yu.,
  • Gagarina I.N.,
  • Gorkova I.V.,
  • Solokhina I.Yu.

DOI
https://doi.org/10.1051/bioconf/20213605007
Journal volume & issue
Vol. 36
p. 05007

Abstract

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Studies have been conducted on the use of an optimized Murashige-Scoog nutrient medium to obtain healthy seeds of promising potato varieties. It was found that the use of agarized modified Murashige-Scoog nutrient medium with the addition of exometabolites of fungi of the genus Trichoderma lixii 1.5 mg/l with antimicrobial properties and the antiviral drug “Cycloferon” in a concentration of 0.01% contributes to an increase in the number of virus-free potato regenerants when the method of apical meristems and in vitro improvement is used together. The effectiveness of using the modified medium is confirmed by the following criteria: active growth of test tube potato micro-plants, which is expressed in optimal morphometric indicators on the example of the potato variety “Rosara”; the size of the isolated meristems is 10 mm, which contributes to the output of healthy plants; the survival rate of regenerating plants. It is shown that when using the modified medium, an average of 35-50% of regenerating plants were obtained, depending on the potato variety, free from M, S viruses.