A comparative cytotoxic study against breast cancer cells with nanoparticles and rods shaped structures

Abstract

The zinc oxide (ZnO) nanostructures are highly influential material and exhibit numerous properties. Numerous physicochemical applications for instance electronic, catalyst, solar cells, hydrogen fuels and energy evolution. Besides the large application in various directions very few reports are available for the cytotoxic evaluations and their compression with nano particles (NPs) and nanrorods (NRs) against the breast (MCF-7) cancer cells. The ZnO-NPs and NRs were produced through solution process in a short time span and were well characterized. The cells viability was tested with MTT and NRU assays. A series of different concentrations (2 μg/mL, 5 μg/mL, 10 μg/mL, 25 μg/ mL, 50 μg/mL, 100 μg/mL and 200 μg/mL) of NRs and NPs were employed against cancer cells for to evaluate the % activity of sustained and non-sustained cells. The morphology of treated and control cells were observed via microscopy respectively. Including this, the genetic studies were also scrutinized, cell-cycle analysis express the upsurge in the apoptotic peak after a 24-h. Quantitative PCR (qPCR) data revealed that the mRNA levels of apoptotic genes such as p53, bax, and caspase-3 were upregulated, whereas bcl-2, an anti-apoptotic gene, was downregulated; therefore, apoptosis was mediated through the p53, bax, caspase-3, and bcl-2 pathways.

Keywords

• Nanoparticle
• Nanorods
• MTT
• NRU
• ROS
• MCF-7