Skrining Aktivitas Antibakteri Bakteriosin dari Minuman Ce Hun Tiau

Abstract

Lactic acid bacteria is one of the beneficial bacteria because it can produce an antimicrobial peptide called bacteriocin. Bacteriocin has been applied as a natural food preservative because it prevents effectively the growth of pathogenic bacteria on food or drink. The aimed of this study was to identify the lactic acid bacteria as producers of bacteriocin from Ce hun tiau which has inhibitory activity againts pathogenic bacteria such as Escherichia coli, Staphylococus aureus and Salmonella typhi. In this study, Lactic acid bacteria were isolated from Ce Hun Tiau using streak plate method on media deman Rogose Sharpe. Screening bacteriocin use disc diffusion method to find out the inhibitory activity of isolates against Escherichia coli, Staphylococus aureus and Salmonella typhi and for confirmation test using proteolytic enzymes. The results showed one isolate of lactid acid bacteria was succesfully isolated from Ce hun tiau, identified as Lactobacillus plantarum. This isolate could inhibit the growth of pathogenic bacteria Escherichia coli, Staphylococus aureus and Salmonella typhi which is a bacteriocin activity.

Keywords

• bakteriosin
• bakteri asam laktat
• ce hun tiau
• bacteriocin
• lactic acid bacteria