Nature Communications (Aug 2020)

ATP-dependent hydroxylation of an unactivated primary carbon with water

  • Christian Jacoby,
  • Sascha Ferlaino,
  • Dominik Bezold,
  • Henning Jessen,
  • Michael Müller,
  • Matthias Boll

DOI
https://doi.org/10.1038/s41467-020-17675-7
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 10

Abstract

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Monooxygenases catalyse the hydroxylation of C-H bonds using oxygen as a co-substrate, which, in turn, is unavailable for anaerobic bacteria. Here, the authors report a three-step reaction cascade involving two hydroxylases and one dehydratase which hydroxylate the C26 methyl group of cholesterol with water as a co-substrate.