Quorum Sensing Activity of Serratia fonticola Strain RB-25 Isolated from an Ex-landfill Site

Abstract

Quorum sensing is a unique bacterial communication system which permits bacteria to synchronize their behaviour in accordance with the population density. The operation of this communication network involves the use of diffusible autoinducer molecules, termed N-acylhomoserine lactones (AHLs). Serratia spp. are well known for their use of quorum sensing to regulate the expression of various genes. In this study, we aimed to characterized the AHL production of a bacterium designated as strain RB-25 isolated from a former domestic waste landfill site. It was identified as Serratia fonticola using matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry analysis and this was confirmed by 16S ribosomal DNA sequencing. High resolution triple quadrupole liquid chromatography-mass spectrometry analysis of S. fonticola strain RB-25 spent culture supernatant indicated the existence of three AHLs namely: N-butyryl-L-homoserine lactone (C4-HSL), N-hexanoyl-L-homoserine lactone (C6-HSL) and N-(3-oxohexanoyl) homoserine-lactone (3-oxo-C6 HSL). This is the first report of the production of these AHLs in S. fonticola.

Keywords

• cell-to-cell communication
• matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF)
• mass spectrometry
• triple quadrupole liquid chromatography mass spectrometry
• N-butyryl-L-homoserine lactone (C4-HSL)
• N-hexanoyl-L-homoserine lactone (C6-HSL)
• N-(3-oxohexanoyl) homoserine-lactone (3-oxo-C6 HSL)