PLoS ONE (Jan 2023)

Association of urinary sex hormones with mood and behavior changes in a community adolescent cohort.

  • Philip Hazell,
  • Ben W R Balzer,
  • Frances Garden,
  • David J Handelsman,
  • Karen Paxton,
  • Catherine Hawke,
  • Rebecca Ivers,
  • S Rachel Skinner,
  • Georgina Luscombe,
  • Katharine S Steinbeck

DOI
https://doi.org/10.1371/journal.pone.0293040
Journal volume & issue
Vol. 18, no. 10
p. e0293040

Abstract

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ObjectiveTo examine the contribution of variation in sex hormone excretion to mood and behavioral changes in adolescent females and males.DesignProspective, longitudinal observational cohort study.MethodsParticipants were 342 volunteers aged 10-12 years living in rural Australia. Urinary estradiol and testosterone levels measured by liquid chromatography-mass spectrometry were obtained at three-month intervals for three years. Integrated measures (area-under-curve) of urinary steroid excretion summarised as absolute and variability during each 12-month period of the study. Psychosocial data were gathered annually with the primary outcome of depressive symptomatology. Secondary outcomes were the other subscales of the Youth Self-Report, impulsive-aggression, sleep habits, and self-harm.Results277 (158 male) participants contributed data over the full duration of the study and could be included in the analyses. In females, analyses of absolute urine hormone levels found no relationship between estradiol and any outcome, but higher testosterone was significantly associated with depression and poorer sleep. Greater variability of both urine estradiol and testosterone was associated with lower total psychopathology, anxious/depressed and social problems scores. Greater variability in urine estradiol was associated with lower attention problems and impulsive aggression in females. In males, higher testosterone and estradiol levels were associated with rule-breaking, and poorer sleep, and no associations were found for gonadal hormone variability for males.ConclusionsLongitudinal measurement of both iso-sexual and contra-sexual gonadal hormones contributes to a more nuanced view of the impact of sex steroids on mood and behavior in adolescents. These findings may enlighten the understanding of the impact of sex steroids during normal male and female puberty with implications for hormone replacement therapies as well as management of common mood and behavioral problems.