Highly simplified method for gas-liquid chromatographic quantitation of bile acids and sterols in human stool

Abstract

A simple method for the gas–liquid chromatographic quantitation of human fecal bile acids and sterols is described where bile acids are subjected to n-butyl ester derivatization, without prior isolation from the stool, followed by trimethylsilylation of the sterols and bile acids. Under these conditions, bile acid derivatives are well resolved from each other and from the trimethylsilyl ether derivatives of fecal sterols and no overlap occurs. The method was shown to be highly reproducible and recoveries were similar to those obtained with other methods used for fecal bile acid analysis. Application of the method for bile acid and sterol analysis in human stool is described.—Batta, A. K., G. Salen, K. R. Rapole, M. Batta, P. Batta, D. Alberts, and D. Earnest. Highly simplified method for gas–liquid chromatographic quantitation of bile acids and sterols in human stool. J. Lipid Res. 1999. 40: 1148–1154.

Keywords

• bile acids
• bile acid n-butyl ester-trimethylsilyl ethers
• capillary gas–liquid chromatography
• human fecal bile acids
• fecal sterols
• fatty acids