Nature Communications (May 2019)

Low potential enzymatic hydride transfer via highly cooperative and inversely functionalized flavin cofactors

  • Max Willistein,
  • Dominique F. Bechtel,
  • Christina S. Müller,
  • Ulrike Demmer,
  • Larissa Heimann,
  • Kanwal Kayastha,
  • Volker Schünemann,
  • Antonio J. Pierik,
  • G. Matthias Ullmann,
  • Ulrich Ermler,
  • Matthias Boll

DOI
https://doi.org/10.1038/s41467-019-10078-3
Journal volume & issue
Vol. 10, no. 1
pp. 1 – 10

Abstract

Read online

The reduction of 2-naphtoyl-CoA to 5,6 dihydro-2-naphtoyl-CoA by 2-naphtoyl-CoA reductase is below the negative redox limit usually encountered in biological hydride transfer. Here, via X-ray crystallography and spectroscopic analysis, the authors elucidated the mechanism behind this.