Hepatic leukemia factor-expressing paraxial mesoderm cells contribute to the developing brain vasculature
Yuta Koui,
Takako Ideue,
Michael Boylan,
Matthew J. Anderson,
Motomi Osato,
Toshio Suda,
Tomomasa Yokomizo,
Yoh-suke Mukouyama
Affiliations
Yuta Koui
Laboratory of Stem Cell and Neuro-Vascular Biology, Cell and Development Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
Takako Ideue
International Research Center for Medical Sciences, Kumamoto University, Kumamoto 860-0811, Japan
Michael Boylan
Cancer and Developmental Biology Laboratory, National Cancer Institute, National Institutes of Health, Frederick, MD 21702, USA
Matthew J. Anderson
Cancer and Developmental Biology Laboratory, National Cancer Institute, National Institutes of Health, Frederick, MD 21702, USA
Motomi Osato
International Research Center for Medical Sciences, Kumamoto University, Kumamoto 860-0811, Japan
Toshio Suda
International Research Center for Medical Sciences, Kumamoto University, Kumamoto 860-0811, Japan
Tomomasa Yokomizo
International Research Center for Medical Sciences, Kumamoto University, Kumamoto 860-0811, Japan
Yoh-suke Mukouyama
Laboratory of Stem Cell and Neuro-Vascular Biology, Cell and Development Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
Recent genetic lineage tracing studies reveal heterogeneous origins of vascular endothelial cells and pericytes in the developing brain vasculature, despite classical experimental evidence for a mesodermal origin. Here we provide evidence through a genetic lineage tracing experiment that cephalic paraxial mesodermal cells give rise to endothelial cells and pericytes in the developing mouse brain. We show that Hepatic leukemia factor (Hlf) is transiently expressed by cephalic paraxial mesenchyme at embryonic day (E) 8.0-9.0 and the genetically marked E8.0 Hlf-expressing cells mainly contribute to the developing brain vasculature. Interestingly, the genetically marked E10.5 Hlf-expressing cells, which have been previously reported to contain embryonic hematopoietic stem cells, fail to contribute to the vascular cells. Combined, our genetic lineage tracing data demonstrate that a transient expression of Hlf marks a cephalic paraxial mesenchyme contributing to the developing brain vasculature. This article has an associated First Person interview with the first author of the paper.
